Isoelectrofocusing in acetate membrane: the method and application. |
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| Authors: | S Harada |
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| Affiliation: | Department of Legal Medicine, Kyorin University School of Medicine, TokyoJapan |
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| Abstract: | Acetate membrane as a new medium for isoelectrofocusing was used in this study. The acetate membrane was specially produced for isoelectrofocusing, and contains a surface active agent. The ampholine solution system and isoelectric condition for this purpose are described. Polymorphic traits as alpha1-antitrypsin in human serum and 6-phosphogluconate dehydrogenase (6-PGD) in human erythrocyte could be identified by this method. The isoelectric patterns and the isoelectric points of these proteins were demonstrated on the acetate membrane. |
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| Keywords: | Address correspondence to: Dr. Shoji Harada Department of Legal Medicine Kyorin University School of Medicine Sinkawa Mitaka Tokyo 181 Japan. |
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