潮汕地区中枢神经系统疾病肠道病毒感染调查

目的对潮汕地区1972年-2003年间临床诊断为中枢神经系统(CNS)疾病的1 605例患者进行肠道病毒(EV)感染调查.方法采集临床诊断为CNS疾病的患者脑脊髓液、血液、咽拭子或粪便标本共l 605份.用Hep-2细胞对标本进行病毒培养;用免疫血清微量中和试验对分离出的毒株进行鉴定;采集患者的双份血清进行中和抗体检测.结果从l 605例CNS疾病患者(包括疑似脊髓灰质炎、脑炎、格林-巴利综合征、脊髓压迫症、脑血管意外、颅内积水或占位和多发性硬化)的各种标本中共检测出4种25个型EV病原541例,EV病毒总检出率为33.7%,患者EV感染型别分布及检出率构成比分别为:脊髓灰质炎病毒(Polio)260例(0.480 6);柯萨奇病毒(Cox)169例(0.3124);埃可病毒(ECHO)57例(0.105 4);新型EV 55例(0.101 6).结论潮汕地区CNS疾病中存在EV感染.不同年份EV的检出率不尽相同,1972年-1979年、1980年-1989年、1990年-1994年这三个时间段里EV检出率以Polio最高;1995年-2003年EV检出率则以Cox最高.随着脊髓灰质炎疫苗的普及接种,1995年后未再发现Po-lio.

Investigation on enterovirus infection of central nervous system in Chaoshan District

Objective To investigate the enterovrius infection among 1 605 cases of central nervous system (CNS) diseases in Chaoshan District from 1972 to 2003. Methods After the samples of cerebral spinal fluid, blood, pharyngeal secretion and stool were collected from the 1 605 patients with CNS diseases, viral culture was performed using Hep -2 cells and viral identification done by neutralization test using the immune serum. Meanwhile, two samples of serum were collected form each patient for detection of neutralizing antibody. Results A total of 25 types of 4 kinds of enteroviruses were detected in 541 patients (3.7%). The clinically diagnosed diseases included poliomyelitis, encephalitis, Guilain - Barre syndrome, myeleterosis, cerebrovascular accident, intracranial hypertension and multiple sclerosis. As for causes of viral infection, po- liovirus was found in 260 cases, Coxsackie virus in 169, ECHO virus in 57 ant enterovirus in 55. Conclusions CNS disea- ses due to enterovirus infection exist in Chaoshan District and different types and kinds of viruses are isolated in different time periods. The infection ratio of poliovirus is the highest from 1972 to 1994 and that of the Coxsackie virus is the highest from 1995 to 2003 (63.3%). No poliovirus has been isolated since 1995 because of generalization of poliovirus vaccine inocula- tion.