环境噪音对新生大鼠身长体质量及学习记忆的影响

目的研究环境噪音对新生大鼠身长体质量及学习记忆的影响。方法选用7 d SD大鼠16只,分为高噪音组和对照组,每组8只,高噪音组的平均噪音为90 db(A)、对照组50 db(A)。分别在P10、P15、P20时进行身长、体质量的测量,P20时利用Morris水迷宫观察学习记忆功能、ELISA检测生长激素,RT-PCR、Western blot观察突触素表达。结果高噪音组身长、体质量低于同年龄对照组大鼠[身长(cm):P10(9.54±0.1)vs(9.98±0.1),P15(10.24±0.1)vs(10.79±0.1),P20(10.59±0.1)vs(11.68±0.1);体质量(g):P10(14.33±0.02)vs(14.51±0.02),P15(15.31±0.02)vs(15.68±0.02),P20(16.63±0.02)vs(16.76±0.02),P<0.05];与对照组比较,高噪音组学习能力下降,生长激素、突触素(synaptophysin,SYP)mRNA、SYP表达水平低于同年龄对照组大鼠[生长激素(pg/ml):(299.7±4.9)vs(504.9±13.14),SYP mRNA:(0.182±0.008)vs(1.056±0.011),SYP:(0.168±0.007)vs(0.432±0.025),P<0.05]。结论高噪音环境下新生SD大鼠的身长、体质量增长缓慢,学习记忆能力下降。

Effect of environmental noise on growth and neuropsychological development of newborn rats

Objective To investigate the effect of environmental noise on the growth and neuropsychological development of newborn rats.Methods Sixteen P7 SD rats were randomly divided into a high noise group and a control group(n=8).The mean noise intensities of the high noise group and the control group were 90db(A) and 50db(A),respectively.The body length and weight of the rats were measured at P10,P15 and P20.The Morris water maze test was applied to observe the learning and memory functions of the rats,ELISA was applied for the detection of growth hormone,and RT-PCR and Western blotting were applied to observe the expression of synaptophysin(SYP) at P20.Results The body length and weight of the rats in the high noise group were lower than those in the control group [body length(cm): P10(9.54±0.1) vs(9.98±0.1),P15(10.24±0.1) vs(10.79±0.1) and P20(10.59±0.1) vs(11.68±0.1);body weight(g): P10(14.33±0.02) vs(14.51±0.02),P15(15.31±0.02) vs(15.68±0.02) and P20(16.63±0.02) vs(16.76±0.02),P<0.05].Compared with the control group,the rats of the high noise group showed decreased learning ability,lower level of growth hormone and lower levels of mRNA and protein expressions of SYP [growth hormone(pg/ml):(299.7±4.9) vs(504.9±13.14),SYP mRNA:(0.182±0.008) vs(1.056±0.011),and SYP:(0.168±0.007) vs(0.432±0.025),P<0.05].Conclusion Slow body length and weight growth and declined learning and memory abilities were observed in newborn SD rats under high noise environment.