支气管哮喘小鼠模型中维生素D相关分子的表达

目的 探讨维生素D相关分子在支气管哮喘(简称哮喘)小鼠模型中的表达及地塞米松的干预效果.方法 用卵白蛋白作为致敏原制备小鼠哮喘模型,随机分为两组(n=6),分别为地塞米松处理组和生理盐水处理组(对照组),收集各组小鼠的支气管肺泡灌洗液和支气管组织,计数总细胞数和白细胞分类数,采用real-time RT-PCR技术检测支气管组织中维生素D3上调蛋白1(VDUP1)、维生素D受体(VDR)和1α羟化酶CYP27B1的mRNA表达水平.结果 哮喘组支气管组织中VDUP1mRNA、VDRmRNA和CYP27 B1mRNA水平分别为(2.74±0.99)、(7.06±4.05)和(3.40±2.16),明显高于对照组(分别为1.01±0.18、1.28±0.76、1.45±1.39,P＜0.05)和地塞米松治疗组(分别为0.94±0.34、0.76±0.18、0.27±0.17,P＜0.01).结论 维生素D相关分子可能参与了哮喘的发病过程.

Expression of several molecules correlated with vitamin D in bronchus of asthmatic mouse

Objective To investigate the expression of several molecules correlated with vitamin D and the effect of dexamethasone on them in bronchus of asthmatic mouse. Methods Eighteen BALB/c mice were divided equally into a control,asthmatic and treated groups. The asthmatic mice were sensitized with ovalbumin. The treated mice were injected with dexamethasone via intraperitoneal. After the challenge, the bronehialalveolar lavage fluid(BALF) was collected for examining the cell number and the level of IL-4, and the bronchus tissue was taken to evaluate the airway inflammation. The expressions of VDUP1 ,VDR and CYP27B1mRNA were measured with real-time RT-PCR. Results The levels of VDUP1mRNA, VDR mRNA and CYP27 B1 mRNA in asthmatic bronchus were significantly higher(2.74 ± 0.99,7.06±4.05,3.40 ± 2.16, respectively) than those in control ( 1.01 ± 0.18, 1.28 ± 0. 76, 1.45 ± 1.39,respectively, P ＜0. 05) and those in treated groups (0.94±0. 34,0. 76±0.18,0. 27±0.17,respectively,P ＜0.01). Conclusions Several molecules correlated with vitamin D may be involved in the physiopathologic process in asthma.