|
|||||
|
|
| PHEMA微孔人工角膜支架材料对成纤维细胞生长的影响 | |
| 引用本文： | 刘磊,刘歆,等.PHEMA微孔人工角膜支架材料对成纤维细胞生长的影响[J].同济大学学报(医学版),2002,23(4):287-288. |
| 作者姓名： | 刘磊 刘歆 |
| 作者单位： | 1. 同济大学附属铁路医院眼科,上海,200072 2. 同济大学生命科学院,上海,200331 3. 同济大学医学院生物教研室,上海,200331 |
| 摘 要： | 目的：研究微孔聚甲基丙烯酸β－羟乙酯（poly(2-hydroxyethl methecylate),PHEMA) 对成纤维细胞生长的影响并与无孔PHEMA材料进行比较。结果：通过含材料浸渍液的培养基培养成纤维细胞来评价微 孔和无孔的PHEMA材料的细胞毒性，分别于24、48和72h用0．5％台盼蓝法对每组进行细胞活力的测定。结果：成纤维细胞在含材料浸渍液的培养基中生长良好，培养24、48和72h后细胞活力与对照组差异无显著性（P＞0．05）；微孔PHEMA与无孔PHEMA之间对细胞活力影响亦无明显差异（P＞0．05）。结论：微孔PHEMA材料具有良好的生物相容性，是理想的人工角膜支架材料。 |
| 关 键 词： | PHEMA 微孔 人工角膜 支架材料 生物相容性 成纤维细胞 |
| 文章编号： | 1008-0392(2002)04-0287-02 |
| 修稿时间： | 2002年1月28日 |
Effects of Porous PHEMA as Karatoprosthesis Skirt on Growth of Fibroblast |
|
| LIU Lei,ZHENG Yi-ren,LIU Xin,SHI Hong-jun.Effects of Porous PHEMA as Karatoprosthesis Skirt on Growth of Fibroblast[J].Journal of Tongji University(Medical Science),2002,23(4):287-288. | |
| Authors: | LIU Lei ZHENG Yi-ren LIU Xin SHI Hong-jun |
| Institution: | LIU Lei 1,ZHENG Yi-ren 1,LIU Xin 2,SHI Hong-jun 3 |
| Abstract: | Objective To evaluate effects of porous PHEMA on growth of fibroblast and compare the biocompatibility of. porous PHEMA and non-porous PHEMA in vitro.Methods Porous PHEMA and non-porous PHEMA were infused in 1640 nutrient media for 24h and fibroblasts were cultured in above media. Cytotoxicity test was carried out after 24,48,and 72 hours.Results The quantity and activity of cells after 24,48 and 72 hours were not significantly different. There was also no difference between the group of porous PHEMA and non-porous PHEMA.Conclusion Porous PHEMA is a good biocompatible material. |
| Keywords: | PHEMA biocompatibility comea manpower |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《同济大学学报(医学版)》浏览原始摘要信息 | |
| 点击此处可从《同济大学学报(医学版)》下载免费的PDF全文 | |