| O139群霍乱弧菌ctxAB基因序列分析 |
| |
| 引用本文： | 王长奇 陈燕萍 等. O139群霍乱弧菌ctxAB基因序列分析[J]. 江西医学检验, 2002, 20(2): 74-76 |
| |
| 作者姓名： | 王长奇 陈燕萍 等 |
| |
| 作者单位： | 江西萍矿职工总医院检验科 337042萍乡(王长奇，陈燕萍，林志铭，伍淑云，罗娜)，江西萍矿职工总医院检验科 337042萍乡(周文峰) |
| |
| 摘 要： | 目的 探讨O130群霍乱JX株的ctxAB基因序列，分析该株基因特点。方法 采用PCR扩增技术，检测含O130群霍乱标本，对ctxAB阳性者进行核苷酸序列测定，分析基因变异情况。结果 发现阳标本中，对其中一株进行了ctxAB基因序列测定，该序列与853（O139群）和N16961（Eltor)同源性为100％，与569B（古典型）同源性99．7％。结论 该研究证明本株与O1群（EVC）遗传关系最近，可能属于B型。
|
| 关 键 词： | O139群霍乱弧菌 序列分析 ctxAB PCR 霍乱 |
| 文章编号： | 1008-0023(2002)02-0074-03 |
Gene sequencing analysis on vibrio cholera O139strains ctxAB of PingXiang. |
| |
| WANG Changqi,CHEN Yanping,LIN Zhimin,et al.. Gene sequencing analysis on vibrio cholera O139strains ctxAB of PingXiang.[J]. Jiangxi Journal of Medical Laboratory Sciences, 2002, 20(2): 74-76 |
| |
| Authors: | WANG Changqi CHEN Yanping LIN Zhimin et al. |
| |
| Affiliation: | WANG Changqi,CHEN Yanping,LIN Zhimin,et al.General Hospital of Pingxiang Mining,Pingxiang337042,China |
| |
| Abstract: | Objective To investigate the ctxAB gene sequence and analyze the characteristics of O139epidemic strains.Mothods PCR was performed to detect the O139VC in the bacteria sample.The positive sample was sequenced and analysed.Re -sults PCR products of one strain in the positive sample were sequenced.The gene se-quences showed100%uncleotide homology to853(O139strains)and N16961Eltor strains,and99.7%to569B (CVC).Conclusion The present study indicates that O139VC and O1EVC in Pingxiang are genetically nearest.The O139VC might belong to type B. |
| |
| Keywords: | Vibrio cholerae O139 Sequence analysis Genes |
| 本文献已被 CNKI 维普 等数据库收录！ |
