| 绵枣儿核核糖体DNA ITS序列扩增的研究 |
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| 引用本文： | 尚彩玲,丁开宇. 绵枣儿核核糖体DNA ITS序列扩增的研究[J]. 山西中医学院学报, 2009, 10(4) |
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| 作者姓名： | 尚彩玲 丁开宇 |
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| 作者单位： | 山西中医学院,山西,太原,030024;云南大学生命科学院,云南,昆明,650091 |
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| 基金项目： | 国家自然基金资助项目 |
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| 摘 要： | 采用通用引物扩增绵枣儿核核糖体DNA ITS序列,未得到目的条带.根据引物设计原则自行设计12个引物,其中只有使用引物对PA3、D(ITS3),PA4、D(ITS3),PA4、DO2扩增得到绵枣儿核核糖体DNA ITS序列.
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| 关 键 词： | 核核糖体 ITS序列 引物设计 |
Study on Nuclear Ribosomal DNA ITS Amplification of Scilla scilloides(Lindl.)Druce |
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| Shang Cailing,Ding Kaiyu. Study on Nuclear Ribosomal DNA ITS Amplification of Scilla scilloides(Lindl.)Druce[J]. Journal of Shanxi College of Traditional Chinese Medicine, 2009, 10(4) |
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| Authors: | Shang Cailing Ding Kaiyu |
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| Affiliation: | 1.Shanxi College of TCM;Taiyuan Shanxi 030024;2.College of Life and Sciences;Yunnan University;Kunming Yunnan 650091 |
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| Abstract: | Universal primers were used to amplify nuclear ribosomal DNA ITS of Scilla scilloides(Lindl.)Druce,but they did not work.Then 12 primers were designed according to the principle of primer-design and were used .Nuclear ribosomal DNA ITS of Scilla scilloides(Lindl.)Druce was obtained using primers PA3 and D(ITS3), PA4 and D(ITS3), PA4 and DO2. |
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| Keywords: | nuclear ribosomal ITS primer design |
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