| 高效液相色谱法测定复方黄连颗粒中葛根素的含量 |
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| 引用本文： | 唐丽琴,刘圣,陈礼明,李矗. 高效液相色谱法测定复方黄连颗粒中葛根素的含量[J]. 中国药房, 2003, 14(7): 425-427 |
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| 作者姓名： | 唐丽琴 刘圣 陈礼明 李矗 |
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| 作者单位： | 安徽省立医院药剂科,合肥市,230001 |
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| 基金项目： | 安徽省重点科研项目 (01023018) |
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| 摘 要： | 目的 :建立复方黄连颗粒中葛根素含量的测定方法。方法 :采用高效液相色谱法 ,色谱柱为C18(4 6mm×250mm ,5μm) ,检测波长为250nm ,流动相为甲醇 -水 (30∶70) ,流速为1 0ml/min ,柱温为30℃。结果 :葛根素在0 312μg～1 560μg范围内线性关系良好 (r=0 9989)。葛根素的平均回收率为99 2 % (RSD=2 08% ,n=5)。结论 :本方法具有灵敏度高 ,操作简单、稳定 ,分离效果好等优点 ,可作为复方黄连颗粒中葛根素的含量测定方法
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| 关 键 词： | 高效液相色谱法 复方黄连颗粒 葛根素 含量 |
| 文章编号： | 1001-0408(2003)07-0425-03 |
| 修稿时间： | 2003-01-13 |
Determination of the Content of Puerarin in Compound Rhizoma Coptidis Graininess by HPLC |
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| TANG Liqin,LIU Sheng,CHEN Liming,LI Chu. Determination of the Content of Puerarin in Compound Rhizoma Coptidis Graininess by HPLC[J]. China Pharmacy, 2003, 14(7): 425-427 |
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| Authors: | TANG Liqin LIU Sheng CHEN Liming LI Chu |
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| Abstract: | OBJECTIVE:To establish a method for determination of the content of puerarin in compound Rhizoma Coptidis graininess METHODS:HPLC method was used The samples were separated by C18 (4 6mm×250mm,5μm) column with the mobile phase of methanol-water(30∶70) The detection wavelength was set at 250nm Flow rate was 1 0ml/min Column temperature was 30℃ RESULTS:The linear ranges of puerarin was 0 312μg～1 560μg The average recovery was 99 2%,RSD=2 08%(n=5) CONCLUSION:The method is sensitive,rapid and accurate and suitable for the determination of puerarin in compound Rhizoma Coptidis graininess |
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| Keywords: | HPLC compound Rhizoma Coptidis graininess puerarin content |
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