胃癌中医证型与C-erbB-2表达水平的相关性研究

目的 检测C-erbB-2基因在胃癌不同中医证型中的表达水平及变化，探讨C-erbB-2基因与胃癌中医证型的关系。方法 选取2012年10月～2014年10月在甘肃省肿瘤医院胃肠外科入院行手术治疗的胃癌患者，以慢性浅表性胃炎（chronic superficial gastritis）或正常胃组织标本为对照组，使用免疫组织化学（immunocytochemistry）的技术检测胃癌组织和正常组织标本中C-erbB-2的表达情况，并在显微镜下观察记录组织细胞的阳性表达水平。结果 共纳入胃癌患者162例，其中女性64例，男性98例，平均年龄53.1±7.4岁；对照组40例，包括女性11例和男性29例，平均年龄44.7±5.3岁。不同类型的中医证型中，C-erbB-2蛋白表达由强到弱依次为：痰湿凝结型（94.1%）> 气滞血瘀型（82.6%）> 肝气犯胃型（75.0%）> 胃热伤阴型（71.4%）> 气血亏虚型（63.2%）>脾胃虚寒型（61.5%），且差异有统计学意义（P<0.05），并与胃癌的分级、分期存在一定的相关性。结论 C-erbB-2基因的表达在不同胃癌中医证型中存在演变趋势。

Correlation Study of TCM Syndrome Types of Gastric Cancer and Level of Expression of C-erbB-2

Objective To detect the level of C-erbB-2 gene in different traditional Chinese medicine (TCM) syndromes type of gastric cancer tissues, and analyse the correlation between expression of C-erbB-2 and TCM syndromes type. Methods We selected 162 patients with gastric cancer, who were experienced surgical resectionin, in Cancer Hospital of Gansu Province from October 2012 to October 2014. The chronic superficial gastritis and normal gastric tissues as control group. The expression of C-erbB-2 in gastric cancer tissues were detected by immunohistochemical methods. Data were analyzed and the results were compared. Results There were 162 patients with gastric cancer enrolled, including 64 cases of female, 98 cases of male, mean age 53.1±7.4 years, the control group 40 cases, including 11 females, 29 males, and mean age 44.7±5.3 years. The expression of C-erbB-2 protein levels in different TCM syndrome types from strong to weak: Phlegm Stagnation (94.1%) > Qi and Blood Stagnation type (82.6%) > Fan Wei Qi type (75.0%) > Stomach Heat Shangyin type (71.4%) > Blood Loss Deficiency syndrome (63.2%) > Spleen and Stomach Cold (61.5%). The positive expression rate of C-erbB-2 in gastric cancer patients is a significant difference between the different type gastric cancer TCM syndromes (P<0.05), and there is a clear correlation between C-erbB-2 and gastric cancer stage. Conclusion The C-erbB-2 expression level of gastric cancer significantly correlated with Phlegm Stagnation type and Qi and Blood Stagnation type, suggesting that C-erbB-2 in gastric cancer have more closely relationship with Shi syndrome type.