Survivin基因在胃癌中表达的研究

目的研究凋亡抑制基因survivin在胃癌组织中的表达。方法运用S-P免疫组化技术,检测survivin基因在93例胃癌组织、20例正常胃黏膜组织以及胃癌细胞株MGC-803中的表达。结果Survivin基因在20例正常胃黏膜组织中不表达,而93例胃癌组织中有66例表达阳性,占71%,两者差异有统计学意义(P<0.01),胃癌细胞株MGC-803中survivin呈高表达。Survivin基因表达阳性率与胃癌组织的分化程度、淋巴结转移、TNM分期相关,而与浸润程度不相关。结论Survivin基因的特异性高表达在胃癌的发生发展中起重要作用,其过度表达可能提示胃癌预后不良。

Survivin gene expression in gastric carcinoma

Objective To detect the expression of a novel inhibitor gene of apoptosis,survivin in gastric carcinoma tissues and in gastric carcinoma cell line MGC-803.Methods In 93 gastric carci-noma tissues and 20 no-neoplastic gastric tissues,the expression of survivin gene was examined by us-ing streptavidin-biotin peroxidase(SP) method.Results In contrast to no expression of survivin in no-neoplastic gastric mucosa tissues,survivin was expressed in 66 of 93(71%) cases of gastric cancer samples,and overexpression of survivin in gastric carcinoma cell line MGC-803 was also presented.Survivin gene expression was related to the differentiation,lymph node metastase and the TNM stage of gastric carcinoma.Conclusions The specifically up-regulation of survivin gene in gastric cancer indi-cates that survivin may play an important role in the process of carcinogenesis.