脉冲电磁场、碱性成纤维细胞生长因子促进坐骨神经延长的实验研究

目的探讨减轻周围神经在缓慢牵拉过程中的损伤，增加神经延长率的方法。方法采用改良的组织扩张器，对大白兔坐骨神经行扩张延长的同时，辅以脉冲电磁场（ＰＥＭＦ）及碱性成纤维细胞生长因子（ｂＦＧＦ）处理。最后，测定神经延长率（ＮＥＲ）、运动传导速度（ＭＣＶ）及组织病理观察。结果ＮＥＲ在ＰＥＭＦ组及ｂＦＧＦ组均显著高于对照组，而ＭＣＶ的降低却显著较轻。组织病理显示，ＰＥＭＦ及ｂＦＧＦ组的神经变性轻，雪旺氏细胞增殖，新生毛细血管及髓鞘却较明显。结论ＰＥＭＦ及ｂＦＧＦ可减轻神经在扩张期间的损伤，增加延长率

Experimental Study on Sciatic Nerve Elongation Enhanced by Pulsed Electromagnetic Field and Basic Fibroblast Growth Factor

Aim To determine the effect of pulsed electromagnetic field (PEMF) and basic fibroblast growth factor (bFGF) on the nerve expansion to lighten the peripheral nerve injury. Methods Thirty adult New Zealand white rabbits were divided randomly into three groups: the control group (group A), the group dealt with PEMF (group B) and the group with bFGF (group C). PEMF or bFGF was applied while the sciatic nerves were expanded. Four weeks later, the nerve elongation rate (NER), and the movement conduction velocity (MCV) were measured and the nerves were observed histopathologically. Results NERs in group B and group C were significantly higher than that in group A ( P <0.05, P <0.02). The decreases of MCV in group B and group C were significantly less than that in group A ( P <0.002, P <0.001). Nerve degeneration was less, but nerve regeneration and vessel proliferation were much more in group B and group C than those in group A in histopathologic aspects. Conclusion PEMF and bFGF can enhance the peripheral nerve elongation and decrease its damage during the peripheral nerve expansion.