多元线性分光光度法测定复合维生素B片

目的：建立复合维生素B片中维生素B1，维生素B1的含量测定方法，方法：采用多波长多元线性回归分光光度法，维生素B1的测定波长为245nm,248nm和251nm,维生素B1的测定波长为258nm,261nm和264nm，溶剂为盐酸液（9→1000），结果：标准曲线在4．0μg/ml20.0μg/ml范围内呈良好线性关系，平均回收率维生素B1为99．85，RSD小于2．21％，维生素B2为98．7％，RSD小鼠1．9％，结论：地可准确测定复合维生素B中两组分的含量。

Determination of vitamin B1 and B2 in compound vitamin B using multiple lincar regression spectrophotometry

objective: To establish the determination of vitamins B1 and B2in compound vitamin B. Methods: The multiple linear regression spectrophotometry was used with wavelength of 245nm, 248nm, 251nm and 258nm, 261nm and 264nm. The solvent was HCL. Results: The calibration curve was linear between 4.0μg/ml to 20.0mg/ml. The relative standard deviations vitamins B1 and B2 were less than 1.1% and 0.7% at the three different concentrations. Their recovery rates was 101.4% and 99.4%. Conclusion: This method is accurate for the determination of vitamins B1 and B2 in the compound vitamin B.