<Emphasis Type="Italic">Brugia malayi</Emphasis> Adult Low Molecular Weight IgG4-Reactive Antigens Induce Differential Cytokine Response in Lymphocytes of Endemic Normal and Asymptomatic Microfilariae Carriers <Emphasis Type="Italic">In Vitro</Emphasis> |
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Authors: | Bimal P Mohanty Ramanuj Lahiri Shailja Misra-Bhattacharya Santosh K Kar |
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Institution: | (1) School of Biotechnology, Jawaharlal Nehru University, New Delhi, 110067, India;(2) Present address: Central Inland Fisheries Research Institute, Barrackpore, Kolkata, 700120, India;(3) Present address: National Hansen’s Disease Program, Louisiana State University, Baton Rouge, Louisiana 70803, USA;(4) Division of Parasitology, Central Drug Research Institute, Lucknow, 226001, India |
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Abstract: | To characterize putatively protective immune response in bancroftian filariasis, Th1/Th2 cytokine profile induced in peripheral
blood mononuclear cells (PBMCs) of endemic normal (EN) and asymptomatic microfilaremic (ASM) individuals were studied using
different molecular weight fractions of Brugia malayi adult soluble antigens (BmA), which are differentially recognized by IgG4 antibodies present in their sera. Infection free
and putatively immune individuals living in a filaria endemic area were identified and included in the present study as EN
only after careful longitudinal follow up for three years. It was observed that the low molecular weight antigens present
in Fr4 and Fr5 induced differential cytokine response; EN individuals showed a strong Th1 bias whereas ASM individuals showed
a strong Th2 bias even though both the groups produced Th1 cytokines, albeit of different quantity, when a nonhelminthic antigen
like H37Rv whole cell lysate was used. Since antigens present in Fr5 induced a highly polarized response, they should be examined
for their diagnostic potential in lymphatic filariasis. |
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Keywords: | bancroftian filariasis longitudinal follow up infection free endemic normal individual Brugia malayi adult soluble antigens IgG4-reactive antigen fraction differential Th1/Th2 cytokine response |
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