三氧化二砷对前列腺癌细胞株PC-3生长影响的研究

目的 :探讨三氧化二砷 (As2 O3 )对雄激素非依赖性人前列腺癌细胞株PC 3生长的影响及其机制。　方法 :通过光学显微镜观察As2 O3 处理前后培养的PC 3细胞生长和形态的变化 ,采用MTT法了解不同浓度As2 O3 作用后PC 3细胞的生长抑制曲线 ,应用流式细胞仪Annexin V FITC/PI双染法分析不同浓度As2 O3 处理的PC 3细胞的凋亡情况。　结果 :As2 O3 作用后 ,PC 3细胞形状变圆 ,体积变小 ,胞质透亮度下降 ,部分细胞脱落悬浮于培养基中。在7.81 2 5、1 5 .6 2 5、31 .2 5 0、6 2 .5 0 0、1 2 5、2 5 0、5 0 0 μmol/LAs2 O3 作用 4 8h和 72h后 ,MTT法检测细胞生长抑制率分别为(0 .0 6± 0 .99)、(1 5 .0 1± 1 .1 2 )、(2 9.2 1± 1 .31 )、(34.32± 1 .1 4 )、(4 0 .5 1± 1 .81 )、(6 9.39± 1 .74 )、(73.1 9± 2 .4 1 ) %和(0 .0 4± 1 .5 1 )、(1 6 .1 9± 1 .0 4 )、(4 3.6 1± 1 .1 2 )、(5 6 .6 6± 1 .2 3)、(73.1 3± 2 .6 1 )、(85 .2 2± 1 .74 )、(91 .4 1± 2 .81 ) %。用流式细胞仪检测经过 0 (对照组 )和 0 .1、1 .0、3.0、5 .0、2 0 .0、5 0 .0 μmol/LAs2 O3 作用 4 8、72h后的PC 3细胞 ,凋亡率分别为 0 .87%、5 .33%、8.94 %、9.6 6 %、1 2 .5 6 %、4 5 .5 9%、6 9.0 9%和 0 .1 3%、1 3.4 9%、

Experimental Research on the Effect of Arsenic Trioxide on the Growth of Prostate Cancer PC-3 Cell Lines

OBJECTIVE: To evaluate the effect of arsenic trioxide (As2O3) on the growth of human androgen-independent prostate cancer PC-3 cell lines. METHODS: The changes of the growth and shape of PC-3 cells cultured in As2O3 of different concentrations were observed under the optical microscope after using different concentrations of As2O3, and the growth inhibition curve of As2O3 was obtained by means of MTT. An analysis was made of the apoptosis of the PC-3 cells treated with different concentrations of As2O3 by flow cytometry. And the inhibiting mechanism of As2O3was investigated by means of Annexin-V-FIFC/PI double staining. RESULTS: Affected by As2O3, the PC-3 cells turned round and small and less transparent in cytoplasm, and some sheded and suspended in the culture medium. Forty-eight hours after treatment with different concentrations of As2O3 (7.8125 micromol/L, 15.625 micromol/L, 31.25 micromol/L, 62.5 micromol/L, 125 micromol/L, 250 micromol/L, 500 micromol/L), the growth inhibition rates of the PC-3 cells were (0.06+/-0.99)%, (15.01+/-1.12)%, (29.21+/-1.31)%, (34.32+/-1.14)%, (40.51+/-1.81)%, (69.39+/-1.74)%, and (73.19+/- 2.41)% respectively; and 72 hours after treatment, the growth inhibition rates were (0.04+/-1.51)%, (16.19+/-1.04)%, (43.61+/-1.12)%, (56.66+/-1.23)%, (73.13+/-2.61)%, (5.22+/-1.74)% and (91.41+/-2.81)%, respectively. Forty-eight hours after the administration of different concentrations of As2O3 (0.1 micromol/L, 1.0 micromol/L, 3.0 micromol/L, 5.0 micromol/L, 20.0 micromol/L, 50.0 micromol/L), the apoptosis rates were 0.87%, 5.33%, 8.94%, 9.66%, 12.56%, 45.59% and 69.09%, respectively; and 72 hours after the administration, the apoptosis rates were 0.13%, 13.49%, 4.96%, 11.10%, 20.72%, 92.92% and 93.61%, respectively. CONCLUSION: As2O3 could inhibit the growth of androgen-independent prostate cancer PC-3 cell lines. The inhibiting effect on PC-3 cell lines is correlated with the time of treatment and concentration of As2O3, and accelerates cell apoptosis.