脑源性神经营养因子相关基因与创伤后应激障碍患者的人格特征关联性

目的:分析脑源性神经营养因子(BDNF)主要传导通路中BDNF、PKB1、GSK-3B、PRKCG基因多态性与创伤后应激障碍(PTSD)康复人群艾森克人格的关联性,探讨其单基因及联合作用对人格的影响。方法:严格按照入组标准筛选创伤后应激障碍患者114例,采用PTSD进行诊断,用艾森克人格问卷(EPQ)测量人格特征。采用聚合酶链反应(PCR)直接测序法检测BDNF、PKB1、GSK-3B以及PRKCG基因的单核苷酸多态性(SNP)。结果:Hardy-Weinberg遗传平衡检验到BDNF、PKBI、GSK-3B、PRKCG 4个基因的6个SNPs,即BDNF rs6265和rs712444,PKBl rs2494746和rs2494738,GSK-3Brs6782799以及PRKCG rs3745406间PTSD患者临床痊愈后所测人格差别无统计学意义。6个SNP基因型分布在总样本中均符合Hardy-weinberg遗传平衡(P0.05),样本具有群体代表性。结论:BDNF rs6265,BDNF rs712444,PKB1rs2494746,PKB1rs2494738,GSK-3Brs6782799,PRKCG rs3745406位点与PTSD患者临床痊愈后所测人格可能无关联。

Relationship between Brain-derived Neurotrophic Factor-related Genes and Personality Traits of Post-traumatic Stress Disorder Patients

Objective:To analyze the association between BDNF,PKB1,GSK - 3B and PRKCG gene polymor-phisms which in the BDNF main conduction pathway and the Eysenck personality in the post-traumatic stress dis-order(PTSD)rehabilitation population,and explore the effect of single gene and combined effect on personality.Meth-ods:114 cases of posttraumatic stress disorder were selected strictly according to the inclusion criteria.PTSD was used to diagnose and personality traits were measured by EPQ(Eysenck Personality Questionnaire).The single nu-cleotide polymorphisms(SNPs)of BDNF,PKB1,GSK-3B and PRKCG genes were detected by polymerase chain re-action(PCR)direct sequencing.Results:In this study,we detected six SNPs of BDNF,PKBI,GSK-3B and PRKCG, which were BDNF rs6265,rs712444,PKB1 rs2494746,rs2494738,GSK- 3B rs6782799 and PRKCG rs3745406 by Hardy-Weinberg genetic balance test.The personality of PTSD patients after clinical recovery showed no significant difference.The SNP genotype distributions accorded with the Hardy-weinberg genetic balance(P >0.05)in the total samples.The samples were representative of the population.Conclusion:BDNF rs6265,BDNF rs712444,PKB1 rs2494746,PKB1 rs2494738,GSK-3B rs6782799,and PRKCG rs3745406 locus may not be associated with person-ality measured after clinical recovery in patients with PTSD.