| 利用毛细管电泳-单链构象多态性快速鉴定血液感染病原菌的研究 |
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| 引用本文： | 刘春江,黄君富,夏涵,张晓莉,王珏,潘虹,府伟灵. 利用毛细管电泳-单链构象多态性快速鉴定血液感染病原菌的研究[J]. 第三军医大学学报, 2010, 32(6) |
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| 作者姓名： | 刘春江 黄君富 夏涵 张晓莉 王珏 潘虹 府伟灵 |
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| 作者单位： | 第三军医大学西南医院检验科,重庆,400038 |
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| 基金项目： | 国家自然科学基金(30672004)~~ |
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| 摘 要： | 目的研究毛细管-单链构象多态性(capillary electrophoresis-single strand conformation polymorphism,CE-SSCP)分析技术鉴定血液感染病原菌的可行性,并建立一种快速检测病原菌的新方法。方法选取7种常见血液感染病原菌,在其16S rRNA的保守区设计通用引物,对16S rRNA基因进行扩增,然后将PCR产物进行CE-SSCP分析,依靠CE-SSCP图谱来鉴定病原菌。结果通用引物能够扩增出7种病原菌;CE-SSCP分析后每种菌具有不同出峰时间:大肠杆菌(3999);金黄色葡萄球菌(4082);铜绿假单胞菌(3958);表皮葡萄球菌(4063);肺炎链球菌(4055);肺炎克雷伯菌(3980);粪肠球菌(4166)。结论采用毛细管电泳-单链构象多态性分析技术可简便、快速地鉴定常见血液感染病原菌。
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| 关 键 词： | 毛细管电泳 单链构象多态性 通用引物 PCR 血液感染 |
Rapid identification of blood pathogens by capillary electrophoresis-based single strand conformation polymorphism |
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| Liu Chunjiang,Huang Junfu,Xia Han,Zhang Xiaoli,Wang Jue,Pan Hong,Fu Weiling. Rapid identification of blood pathogens by capillary electrophoresis-based single strand conformation polymorphism[J]. Acta Academiae Medicinae Militaris Tertiae, 2010, 32(6) |
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| Authors: | Liu Chunjiang Huang Junfu Xia Han Zhang Xiaoli Wang Jue Pan Hong Fu Weiling |
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| Affiliation: | Department of Laboratory Medicine;Southwest Hospital;Third Military Medical University;Chongqing;400038;China |
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| Abstract: | Objective To investigate the feasibility of identifying pathogens in blood infection by capillary electrophoresis-based single strand conformation polymorphism (CE-SSCP), and to establish a new method for rapid identification of pathogens. Methods A pair of universal primers was designed and synthesized according to the sequences of 16S rRNA of 7 stains of blood pathogens, including E.coli, S. aureus, P. aeruginosa, S. epidermidis, S. pneumoniae, K. pneumoniae, and E. faecalis. The genomic DNA of above stra... |
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| Keywords: | capillary electrophoresis single strand conformation polymorphism universal primer blood infection |
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