紫杉醇诱导MCF-7乳腺癌细胞凋亡的蛋白质组研究

目的 研究紫杉醇诱导MCF-7乳腺癌细胞凋亡的分子机制。方法 以100nmol／L紫杉醇作用MCF-7细胞24h后提取细胞总蛋白,利用蛋白质组技术,对紫杉醇诱导的MCF-7乳腺癌细胞凋亡前后的细胞蛋白进行二维电泳图谱分析,通过质谱鉴定差异蛋白质。结果 通过对凋亡前后的蛋白质组二维电泳图谱分析,找到了17个差异较大的蛋白质,其中6个蛋白质通过质谱得到了鉴定,它们是烯醇化酶、细胞核氯离子通道蛋白、角蛋白8、核糖体蛋白S12、galectin-1和Hint。结论 通过蛋白质组技术,发现了在紫杉醇诱导MCF-7乳腺癌细胞凋亡前后发生变化的6种蛋白质;这些差异蛋白质在紫杉醇诱导细胞凋亡中发挥一定的作用。

Proteomic analysis of paclitaxel-induced apoptosis in MCF-7 human breast carcinoma cells

Objective To investigate the mechanism of paclitaxel-induced apoptosis in MCF-7 human breast carcinoma cells.Methods In this study,the proteins extracted from paclitaxel-induced apoptotic MCF-7 cells were analyzed by 2-dimentional gel electrophoresis(2-DE),and compared with those from untreated MCF-7 cells.The differential proteins were identified by mass spectrometry.Results At 24 hour after paclitaxel(100 nmol/L)treatment,MCF-7 cells were collected and extracted the whole proteins. Seventeen up-regulated or down-regulated proteins were found by analysis of the differential proteomic 2-DE map.Six of them were identified by mass spectrometry.They were enolase 1,chloride intracellular channel 1,keratin 8,ribosomal protein S12,galectin-1 and histidine triad nueleotide binding protein,respectively. Conclusion We effectively found the changed proteins in the process of paclitaxel-indueed apoptosis in MCF-7 human breast carcinoma cells by proteomic techniques.These up-regulated or down-regulated proteins are important molecules for our further research about the mechanism of paclitaxel-induced apoptosis.