Steroidogenesis in the blue crab Callinectes sapidus Rathbun |
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Authors: | R K Tcholakian K B Eik-Nes |
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Institution: | 1. Division of Biochemistry &; Physiology of Reproduction, University of Southern California Medical School, Los Angeles, California 90033 USA;2. Department of Physiology, University of Southern California Medical School, Los Angeles, California 90033 USA |
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Abstract: | When radioactive progesterone was incubated with homogenates of androgenic glands from blue crabs, radioactive 20α-hydroxyprogesterone, Δ4-androstenedione, testosterone, and 11-deoxycorticosterone were formed. Per gram tissue the androgenic gland converted more radioactive progesterone to radioactive testosterone and 11-deoxycorticosterone than homogenates of posterior vas deferens or hepatopancreas. Following intrathoracic injection of radioactive Δ5-pregnenolone to male crabs, the above-indicated progesterone metabolites could be isolated and identified. The data are discussed with regard to endogenous production of steroids in the blue crab. |
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