深圳市宝安区石岩街道已婚妇女高危人乳头瘤病毒感染情况调查

目的调查深圳市宝安区石岩街道已婚妇女人乳头瘤病毒(human papilloma virus,HPV)感染以及常见的11种亚型的分布情况。方法取宫颈口分泌物标本,样本脱氧核糖核酸(deoxyribonucleic acid,DNA)的提取按试剂盒说明书进行。采用7500型荧光聚合酶链反应(polymerase chain reaction,PCR)仪扩增及分析。每次实验按试剂盒提供的定性阳性标准和定量阳性标准品做标准曲线,并作出定量分析。同时设置阴性、空白对照。诊断结果呈阳性的标准为:标本检测出的HPV DNA≥5.00E+02拷贝。结果 12 804例女性中检出HPV阳性者2 856例,检出率为22.31%。年龄在22~29岁、30~39岁、40~49岁、50～59岁、≥60岁5组患者HPV感染率分别为29.20%、23.04%、19.88%、20.01%和19.00%。22～29岁年龄段HPV感染率同其他年龄组的感染率相比明显升高,差异有统计学意义(P0.01)。各年龄组HR-HPV所占比例分别为57.30%、82.21%、81.43%、72.12%和66.32%。30~39岁、40~49岁年龄组HR-HPV感染率较其他年龄组明显升高。结论 30岁以下年轻患者的HPV感染率明显高于其他年龄组,30~50岁之间的女性HR-HPV感染率明显增高。

Surveys of Married Women at High-risk Human Papilloma Virus Infection in Shiyan Street of Bao'an District in Shenzhen

Objective To investigate the prevalence of human papilloma virus(HPV) infection and the distribution of 11 subtypes in married women in Shiyan street of Bao’an district in Shenzhen.Methods The cervical secretions from 12 804 married women were collected.Deoxyribonucleic acid(DNA) in the samples was isolated using standard technique and the analyzed with via fluorogenic quantitative polymerase chain reaction(FQ-PCR) according to the manufacturer’s protocol.It was considered as a positive standard if HPV DNA≥5.00E+02 copies.Results The overall infection rate of HPV was 22.31%(2 856/12 804).The infection rate of HPV was 29.20% in females aged 22 to 29 years,23.04% in females aged 30 to 39 years,19.88% in females aged 40 to 49 years,20.01% in females aged 50 to 59 years,and 19.00% in females aged 60 and over.And the prevalence in group aged 22 to 29 years was significantly higher than other groups(P<0.01).The infection rate of high risk(HR)-HPV was 57.30 %,82.21 %,81.43 %,72.12 % and 66.32 % respectively,and the infection rates of the 30 to 39 yesrs old group and 40 to 49 years old group were higher than that of other groups.Conclusion Rate of HPV infection in patients that under 30 years old group is significantly higher than that other ages groups.Infection rates of HR-HPV in females aged 30 to 50 year old is significantly increased.