2型糖尿病患者胰高糖素受体基因Gly40Ser突变的检测

为探讨中国人２型糖尿病与胰高糖素受体（ＧＣＧ－Ｒ）基因４０号密码子的错义突变（Ｇｌｙ４０Ｓｅｒ）是否存在关联，运用聚合酶链反应－限制性片段长度多态性（ＰＣＲ－ＲＦＬ－Ｐ）分析方法在１６０例无亲缘关系之中国云南昆明地区汉族人（２型糖尿病患者１００例，健康对照者６０例）中对ＧＣＧ－Ｒ基因Ｇｌｙ４０Ｓｅｒ突变进行了检测。结果显示：所有研究对象中无论是２型糖尿病患者还是健康对照者均无一例存在ＧＣＧ－Ｒ基因

Screening for the Presence of the Gly40Ser Mutation in the Glucagon Receptor Gene in Patients with Type 2 Diabetes

In order to probe into the relationship between the Gly40Ser mutation in the glucagon receptor(GCG-R) gene and type 2 diabetes mellitus in Chinese, 100 unrelated patients with type 2 diabetes and 60 nondiabetic control individuals without family history of diabetes were screened for the presence of the Gly40Ser missense mutation in the GCG-R gene by means of polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) in a Chinese population from Kunming. The Gly40Ser mutation was not found in any of the 160 Chinese subjects studied. All of the subjects, including type 2 diabetics and the controls, were the homozygous individuals with wild-type genotype Gly40/Gly40. No differences in the frequencies of allele Gly40 and allele Ser40 were observed between diabetic patients and control persons. The results suggested that Gly40Ser variation in the GCG-R gene is not associated with type 2 diabetes in Chinese.