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| p21~(waf1)基因转染对乳腺癌细胞增殖活性的影响 | |
| 引用本文： | 宋洪生,贾伟丽,种瑞峰,李福年,刘相萍,王新刚,王宇.p21~(waf1)基因转染对乳腺癌细胞增殖活性的影响[J].中华乳腺病杂志（电子版）,2008,2(5). |
| 作者姓名： | 宋洪生 贾伟丽 种瑞峰 李福年 刘相萍 王新刚 王宇 |
| 作者单位： | 1. 青岛大学医学院附属医院乳腺病诊疗中心,青岛,266003 2. 青岛大学医学院附属青岛市立医院,青岛,266011 3. 青岛大学医学院附属医院分子生物实验室,青岛,266003 |
| 摘 要： | 目的 探讨p21waf1基因转染对人乳腺癌细胞增殖活性影响及意义.方法 应用脂质体介导法将pIRES-p21waf1真核表达载体转染入人乳腺癌MCF-7细胞系,并得到稳定的表达.通过荧光定量PCR、Western blot法分别检测p21waf1基因表达及蛋白表达情况, MTT法检测细胞增殖,流式细胞仪检测细胞周期分布.结果 p21waf1基因转染后乳腺癌细胞生长显著受抑制,细胞周期停滞于G1期,转染组G1期、S期和G2/M期细胞比例分别为(70.52±3.21)%、(16.38±1.17)%、(9.42±0.98)%,未转染组则为(49.84±1.58)%、(36.83±1.36)%、(15.64±1.09)%,二者相比差异有统计学意义.结论 p21waf1基因转染能够抑制人乳腺癌细胞周期演进和增殖活性,进而阻止乳腺癌的发生、发展,为乳腺癌治疗的一种新手段. |
| 关 键 词： | 基因转染 细胞增殖 乳腺癌 |
Effect of p21~(waf1) gene transfection on the proliferation of human breast carcinoma cells |
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| SONG Hong-sheng,JIA Wei-li,CHONG Rui-feng,LI Fu-nian,LIU Xiang-ping,WANG Xin-gang,WANG Yu.Effect of p21~(waf1) gene transfection on the proliferation of human breast carcinoma cells[J].Chinese Journal of Breast Disease(Electronic Version),2008,2(5). | |
| Authors: | SONG Hong-sheng JIA Wei-li CHONG Rui-feng LI Fu-nian LIU Xiang-ping WANG Xin-gang WANG Yu |
| Institution: | SONG Hong-sheng,JIA Wei-li,CHONG Rui-feng,LI Fu-nian,LIU Xiang-ping,WANG Xin-gang,WANG Yu. Department of Breast Surgery,Affiliated Hospital of Qingdao University Medical College,Qingdao 266003,China |
| Abstract: | Objective To explore the role of p21waf1 gene transfection in the proliferation of human breast carcinoma cells. Methods p21waf1 gene packaged with liposome was transfected into the cells of a human breast carcinoma cell line MCF-7,which stably expressing p21waf1gene. The cell growth was detected by MTT. The cell cycle pattern was assayed by flowcytometry. Results After transfection of pIRES-p21waf1, p21waf1 mRNA and the protein of MCF-7-p21waf1 were highly expressed compared with control. The cell growth w... |
| Keywords: | p21waf1 |
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