粉防己碱脂质体的制备及其稳定性研究

目的:比较粉防己碱脂质体2种制备方法。方法:采用硫酸铵梯度法和薄膜分散法制备粉防己碱脂质体,从渗漏率、粒径大小、磷脂含量3个方面进行稳定性比较,从包封率方面进行质量比较。结果:硫酸铵梯度法制备的粉防己碱脂质体包封率高达81.1%,且稳定性好;薄膜分散法包封率仅为32.9%,且稳定性欠佳。结论:硫酸铵梯度法较薄膜分散法制备粉防己碱脂质体更优。

Preparation and Stability of Tetrandrine Liposomes

OBJECTIVE： To draw a comparison between the two preparative technologies of tetrandrine liposomes. METHODS： Tetrandrine liposomes were prepared with ammonium sulfate transmembrane gradient method and film dispersion method respectively. Leaking ratio, particle size, and content of phospholipid of liposomes were selected as the parameters to indicate the stability of liposomes, and the entrapment efficiency was taken as quality index to compare the two preparation technologies. RESULTS： Tetrandrine liposomes prepared with ammonium sulfate transmembrane gradient technology achieved high entrapment efficiency of 81.1% and good stability, while those prepared by film dispersion method had an entrapment efficiency of only 32.9% with poor stability. CONCLUSION： Ammonium sulfate transmembrane gradient method is feasible and superior to the film dispersion method in the preparation of tetrandrine liposome.