| 气道应用T-bet重组腺病毒抑制哮喘小鼠过敏性气道炎症 |
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| 引用本文： | 陈吉泉,黄海,唐昊,臧远胜,修清玉.气道应用T-bet重组腺病毒抑制哮喘小鼠过敏性气道炎症[J].免疫学杂志,2009(5). |
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| 作者姓名： | 陈吉泉 黄海 唐昊 臧远胜 修清玉 |
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| 作者单位： | 第二军医大学长征医院呼吸内科; |
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| 基金项目： | 国家自然科学基金资助项目(30200116) |
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| 摘 要： | 目的探讨气道应用T-bet重组腺病毒(AdT-bet)对哮喘小鼠气道炎症反应的影响及机制。方法C57BL/6小鼠随机分为4组,实验组(A组)、对照病毒组(B组)、PBS对照组(C组)和正常对照组(D组),A、B、C组采用卵蛋白(OVA)、明矾建立哮喘模型,分别于第19天激发前气道内单次应用50μLAdT-bet(108pfu)、AdLacZ、PBS。26d取肺泡灌洗液(BALF)测定细胞成分、IL-4、IL-5、IFNγ浓度,取血测定血浆IgE水平,观察肺组织病理学变化及GATA-3表达。结果1)A组BALF中的嗜酸粒细胞(EOS)为(0.5±0.2)%明显低于B组(21.2±6.9)%和C组(20.9±6.8)%(P<0.01);2)A组BALF中的IL-4(6.7±3.8)pg/mL和IL-5(12.4±4.9)pg/mL的水平明显低于B组IL-4(91.4±22.5)pg/mL和IL-5(55.6±10.6)pg/mL]和C组IL-4(89.8±23.6)pg/mL和IL-5(56.7±11.5)pg/mL](P<0.01),而IFNγ的水平(710±45)pg/mL则明显高于B组(13.1±3.5...
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| 关 键 词： | 支气管哮喘 T-bet GATA-3 重组腺病毒 气道 |
Inhibition of airway inflammation in asthma mice by intratracheal administration of recombinant T-bet Adenovirus |
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| CHEN Jiquan,HUANG Hai,TANG Hao,ZANG Yuansheng,XIU Qingyu.Inhibition of airway inflammation in asthma mice by intratracheal administration of recombinant T-bet Adenovirus[J].Immunological Journal,2009(5). |
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| Authors: | CHEN Jiquan HUANG Hai TANG Hao ZANG Yuansheng XIU Qingyu |
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| Institution: | CHEN Jiquan,HUANG Hai,TANG Hao,ZANG Yuansheng,XIU Qingyu Department of Respiratory Medicine,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China |
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| Abstract: | Objective To investigate the preventive and therapeutic effects of recombinant T-bet Adenovirus(AdT-bet)on airway inflammation in mice with asthma and the relevant mechanisms.Methods C57BL/6 mice were randomly divided into 4 groups with 12 mice every group.The asthmatic model was established in group A,B,and C by immunization with alum-absorbed ovalbumin.Mice in group A,B,and B were received 50 uL AdT-bet,AdLacZ,and PBS intratracheally on day 19 prior to ovalbumin challenge,respectively.Bronchoalveolar lava... |
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| Keywords: | Asthma T-bet GATA-3 recombinant adenovirus intratracheal |
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