卵巢癌患者血浆miR-205、miR-212及miR-429的水平分析及意义

目的 探讨卵巢癌患者血浆miR-205、miR-212及miR-429水平，分析3者与卵巢癌临床病理学特征的关系。方法 收集本院收治的71例上皮性卵巢癌患者未经治疗前的血浆标本（上皮性卵巢癌组），采用实时定量RT-PCR（qRT-PCR）法检测以上标本的miR-205、miR-212及miR-429水平，收集卵巢癌患者的临床病理学参数（年龄、临床分期、分化程度、组织类型及淋巴结转移情况），比较不同miR-205、miR-212及miR-429水平的临床病理参数分布差异，采用受试者工作特征曲线（ROC）评价血浆miR-205、miR-212及miR-429水平在卵巢癌诊断中的临床价值。同时选取同期的68例女性健康体检者（健康对照组）及66例良性卵巢肿瘤患者（良性卵巢肿瘤组）的血浆标本作对照。结果 上皮性卵巢癌组的血浆miR-205水平高于良性卵巢肿瘤组和健康对照组，但miR-212和miR-429水平低于良性卵巢肿瘤组和健康对照组，差异均有统计学意义（P<0.05）；上皮性卵巢癌组中的miR-205水平与年龄、临床分期、分化程度及淋巴结转移有关，miR-212水平与临床分期、分化程度有关，miR-429水平与临床分期、淋巴结转移有关，以上差异均有统计学意义（P<0.05）；卵巢癌患者血浆miR-205水平与miR-212及miR-429均呈负相关（r=-0.572、-0.325），miR-212与miR-429呈正相关（r=0.473），差异均有统计学意义（P<0.05）；血浆miR-205、miR-212及miR-429诊断卵巢癌的AUC、灵敏度和特异度分别为0.915、92.1%和86.2%，0.944，87.3%和91.0%，0.905、88.5%和83.6%。结论 卵巢癌患者血浆中miR-205呈高表达，miR-212和miR-429呈低表达，与临床病理学参数有关，且在卵巢癌诊断中有一定的价值，可用于卵巢癌的辅助诊断和病情评估。

Expression and clinical value analysis of plasma miR-205, miR-212 and miR-429 in ovarian cancer

Objective To explore the levels of plasma miR-205， miR-212 and miR-429 in ovarian cancer and analyze the relationship between the above indicators and clinical pathology parameters of ovarian cancer. Methods Plasma samples from 71 ovarian cancer patients before treatment were collected. The real-time quantitative RT-PCR （qRT-PCR） was used to detect the levels of miR-205， miR-212 and miR-429， and the clinical pathology parameters of ovarian cancer （age， clinical stage， degree of differentiation， histological type and lymph node metastasis） were collected. The clinical pathology parameters of patients with different levels of miR-205， miR-212 and miR-429 were compared. The receiver operating characteristic curve （ROC） was employed to analyze the clinical value of plasma miR-205， miR-212 and miR-429 in the diagnosis of ovarian cancer. Meanwhile， the plasma samples from 68 healthy volunteers （healthy control group） and 66 patients with benign ovarian tumor （benign group） were collected as control. Results There were higher levels of miR-205 and but lower miR-212 and miR-429 in ovarian cancer group versus other two groups with significant difference （P all<0.05）. In ovarian cancer， the level of miR-205 was related with age， clinical stage， degree of differentiation and lymph node metastasis， and miR-212 was related with clinical stage and degree of differentiation， and miR-429 was related with clinical stage and lymph node metastasis （P all<0.05）. The plasma level of miR-205 was negatively correlated with miR-212 and miR-429 （r=-0.572、-0.325）， and miR-212 was positively correlated with miR-429 with statistically significance （r=0.473，P<0.05）. The AUC， sensitivity and specificity of plasma miR-205， miR-212 and miR-429 in the diagnosis of ovarian cancer were 0.915， 92.1% and 86.2%， 0.944，87.3% and 91.0%， and 0.905， 88.5% and 83.6%. Conclusion There was higher expression of miR-205 but lower expression of miR-212 and miR-429. The plasma levels of the above indicators were related with clinical pathology parameters， showing a certain value in the diagnosis of ovarian cancer.