| 高效液相色谱法测定金鸡片中穿心莲内酯和脱水穿心莲内酯含量 |
| |
| 引用本文： | 张火旺,谢雄瑞,黄湖斌.高效液相色谱法测定金鸡片中穿心莲内酯和脱水穿心莲内酯含量[J].中国药业,2010,19(8):36-37. |
| |
| 作者姓名： | 张火旺 谢雄瑞 黄湖斌 |
| |
| 作者单位： | 1. 广东省河源市药品检验所,广东河源,517000
2. 广东药学院中药学院,广东广州,510006 |
| |
| 摘 要： | 目的 建立金鸡片中穿心莲内酯和脱水穿心莲内酯的含量测定方法。方法色谱柱为HypersilC18（250mm×4．6mm，5μm），甲醇-水（50：50）为流动相，检测波长为225nm和250nm，流速为0．8mL／min，进样量10μL。结果穿心莲内酯、脱水穿心莲内酯的进样量分别在0．02068～0．12408μg和0．08104～0．48624μg范围内与峰面积积分值呈良好的线性关系，平均回收率分别为98．22％和99．13％，RSD分别为1．06％和1．45％（n=6）。结论该方法简便、快速，两组分分离度好，测定结果准确可靠，可作为该制剂的质量控制方法。
|
| 关 键 词： | 金鸡片 穿心莲内酯 脱水穿心莲内酯 高效液相色谱法 |
Content Determination of Andrographolide and Dehydroandrographolide in Jinji Tablets by HPLC |
| |
| Zhang Huowang,Xie Xiongrui,Huang Hubin.Content Determination of Andrographolide and Dehydroandrographolide in Jinji Tablets by HPLC[J].China Pharmaceuticals,2010,19(8):36-37. |
| |
| Authors: | Zhang Huowang Xie Xiongrui Huang Hubin |
| |
| Institution: | ( Heyuan Institute for Drug Control, Heyuan, Guangdong, China 517000; 2. College of TCM, Guangdong Pharmaceutical University, Guangzhou, Guangdong, China 510006) |
| |
| Abstract: | Objective To establish a HPLC method for the content determination of andrographolide and dehydroandrographolide in Jinji Tablets. Methods The Hypersil C18 column（250 mm×4.6 mm, 5 μm） was used. The mobile phase consisted of methanol-water （50：50）. The UV detection wavelength was 225 nm and 250 nm. The flow rate was 0. 8 mL/min, the injection volume was 10 μL. Results The linear range was 0.020 68-0. 124 08μg for andrographolide and 0.081 04-0.486 24 μg for dehydroandrographolide. The average recovery rates were 98.22% and 99. 13%, RSD was 1.05% and 1.46% （n=6）,respectively. Conclusion The proposed method appears to be simple, rapid, sensitive and accurate. It is available to the quality control of Jinji Tablets. |
| |
| Keywords: | Jinji Tablets andrographolide dehydroandrographolide HPLC |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
