舒肝方对心理应激肝郁大鼠神经生殖内分泌的作用

目的:探讨舒肝方(柴胡、芍药、枳壳等)对心理应激肝郁雌性大鼠神经生殖内分泌的影响。方法:以夹尾激怒法建立心理应激肝郁证大鼠模型,检测疏肝补肾中药对大鼠下丘脑垂体和血清NE、DA、5-HT、HVA、DOPAC、5-H IAA以及血清LH、FSH、E2、T、P、PRL水平。结果:模型大鼠下丘脑垂体NE减少,DA、5-HT及5-H IAA含量增加,血清FSH、LH、E2、P降低,NE、DA、5-HT、5-H IAA及T水平升高(P<0.05,P<0.01)。舒肝补肾组血清P及LH水平明显升高;舒肝及舒肝补肾方均能升高模型大鼠下丘脑垂体NE及血清FSH、E2水平,降低下丘脑垂体DA、5-HT、5-H IAA及血清NE、5-HT、T水平;补肾方亦能抑制下丘脑垂体NE、血清FSH、P水平下降及下丘脑垂体与血清5-HT、血清NE、T水平升高。(P<0.05)。结论:心理应激状态下肝郁证雌性大鼠的神经生殖内分泌功能紊乱,舒肝补肾中药具有改善神经生殖内分泌功能的作用。

Infulence of regulating liver-qi and tonifying kidney recipes on hypothalamus-pituitary-ovary axis in rats under psychologic stress

AIM: To investigate the effect of relieving liver-qi recipes(Radix Bupleuri,Radix Paeoniae Alba,Fructus Aurantii,etc.) on the neuro reproductive endocrine in female rats under psychologic stress. METHODS: The psychologic stress induced syndrome of liver-qi stagnation in female rats was established by tail pinch.The hypothalamus pituitary and serum levels of NE,DA,5 HT,5-HIAA,DOPAC,HVA and the serum FSH,LH,E2,T,P,PRL in rats of any groups were measured. RESULTS: The levels of NE in hypothalamus pituitary and FSH,LH,E2,P in serum of model rats were lower and the contents of DA,5-HT,5-HIAA in hypothalamus pituitary and in serum and NE,T in serum were significantly higher than those of the normal control.The serum P and LH in the complex group were elevated.The descending NE in hypothalamus pituitary and FSH,E2 in serum increased and the ascended DA,5-HT,5-HIAA in hypothalamus pituitary and NE,5-HT,T in serum significantly descresed in the shugan group and the complex group.The enhance of NE in hypothalamus pituitary and FSH,P in serum and the reduction of 5-HT in hypothalamus pituitary and 5-HT,NE,T in serum were also obversed in the bushen group. CONCLUSION: Regulating liver-qi and tonifying the kidney recipes could improve the function of neuro reproductive endocrine system which could be attenuated or disturbed by psychologic stress.