结直肠癌组织cripto基因mRNA水平及临床意义 |
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引用本文: | 范钰,郑树,丁佳选,王崇强,张振水.结直肠癌组织cripto基因mRNA水平及临床意义[J].中国临床实用医学,2008,2(2):12-14. |
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作者姓名: | 范钰 郑树 丁佳选 王崇强 张振水 |
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作者单位: | 1. 212002镇江,江苏大学附属人民医院肿瘤研究所,化疗科 2. 浙江大学肿瘤研究所 3. 广东省佛山市中医院放射科 |
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基金项目: | 江苏省镇江市社会发展基金,广东省佛山市卫生厅科研项目 |
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摘 要: | 目的检测cripto基因mRNA在结直肠癌及其癌旁组织中mRNA表达情况,并探讨其在结直肠癌发生发展中的意义。方法提取36例人大肠癌及癌旁正常组织中总mRNA,采用实时荧光定量PCR法检测其中cripto mRNA表达情况;并分析其表达与患者淋巴结转移、肿瘤分化程度和Dukes’s分期相关性。结果cripto mRNA表达在大肠癌组织中阳性表达率为86.1%(31/36),而正常肠组织未见cripto mRNA表达,统计学差异显著(P〈0.0001)。criptomRNA表达与浆膜侵袭、淋巴结转移和Duke’s分期密切相关(P〈0.05,P〈0.05,P〉0.05),而与患者肿瘤发生部位和分化程度无关(P〉0.05,P〉0.05)。结论cripto基因在结直肠癌组织中高表达,可能与大肠侵袭痈转移有关。
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关 键 词: | 结直肠肿瘤 cripto 荧光实时定量PCR 意义 |
Clinical significance of cripto mRNA in human colorectal carcinoma |
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Institution: | FAN Yu,ZHENG Shu,DING Jia-yi.(Cancer Institute, Department of Chemotherapy, Affiliated People's Hospital, Jiangsu University, Jiangsu Zhenjiang 212002, China) |
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Abstract: | Objective To investigate the expression and significance of eripto in human eoiorectal car- cinoma. Methods The expression of eripto mRNA in 36 samples of colon cancer tissues and adjacent normal tissues were determined by real time RT-PCR assay,and 2-△△Ct≥2 as a positive standard. Results The positive rate of cripto expression in coiorectal carcinoma tissue is 86. 1% , however, tumor adjacent tissue is 0. Cripto mR- NA was related with scrosa invasion,lymph node metastasis and Duke's stage,but not with tumor location,and pathological grading. Conclusion Cripto is closely correlated to colorectal carcinoma progression, and cripto might be a potential marker for metastasis of colon cancer progression. |
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Keywords: | cripto |
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