| 非水相固定化酵母催化的(S)-2-辛醇的不对称合成 |
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| 引用本文： | 李泳宁,石贤爱,孟春,董亚琴,郭养浩. 非水相固定化酵母催化的(S)-2-辛醇的不对称合成[J]. 药物生物技术, 2006, 13(6): 446-450 |
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| 作者姓名： | 李泳宁 石贤爱 孟春 董亚琴 郭养浩 |
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| 作者单位： | 福州大学,生物科学与工程学院,福建,福州,350002 |
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| 基金项目： | 福建省自然科学基金;福建省教育厅科研项目 |
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| 摘 要： | 研究固定化面包酵母FD-12对不同水／有机溶剂两相体系的耐受性及不对称合成（S）-2-辛醇的还原反应。结果表明，固定化面包酵母FD-12在水／正十二烷两相体系中的耐受性最好。糖代谢活性保留值可达99.59％。不同的水／有机溶剂两相体系对生物转化影响较大。对反应条件进行优化，在水／正十二烷相体积比为20／5，固定化细胞用量为15．0g，初始底物2-辛酮浓度为200mmol／L的条件下转化96h，（S）-2-辛醇产物得率可达29．59％，ee（S）值达98．81％。
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| 关 键 词： | 非水相 固定化酵母 (S)-2-辛醇 不对称合成 |
| 文章编号： | 1005-8915（2006）06-0446-05 |
| 收稿时间： | 2006-03-22 |
| 修稿时间： | 2006-03-222006-05-08 |
Asymmetric Synthesis of (S)-2-Octanol by Immobilized Yeast in Organic Solvent System |
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| LI Yong-ning,SHI Xian-ai,MENG Chun,DONG Ya-qin,GUO Yang-hao. Asymmetric Synthesis of (S)-2-Octanol by Immobilized Yeast in Organic Solvent System[J]. Pharmaceutical Biotechnology, 2006, 13(6): 446-450 |
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| Authors: | LI Yong-ning SHI Xian-ai MENG Chun DONG Ya-qin GUO Yang-hao |
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| Affiliation: | School of Biological Science and Technology, Fuzhou University, Fuzhou 350002, China |
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| Abstract: | Immobilized baker's yeast FD-12 was employed to the asymmetric synthesis of(S)-2-octanol in the different water/organic solvent biphasic systems.The results showed that the maximal tolerance of immobilized FD12 was observed in water/n-dodecane biphasic system with the value of metabolic activity retention being 99.59%.The experimental data also indicated that the reaction was strongly influenced by the organic solvents selected in the biphasic systems.Under the optimal conditions,that is,(15()g) immobilized cells in water/n-dodecane biphasic system with the Vaq/Vorg being 20/5,by adding 200()mmol/L 2-octanone,a yield of 29.59% and an e.e.of 98.81% of(S)-2-octanol were obtained in(96()h.) |
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| Keywords: | Organic solvent system Immobilized yeast (S)-2-octanol Asymmetric synthesis |
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