| HPLC法测定复方紫黄乳膏中盐酸小檗碱的含量 |
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| 引用本文： | 叶光明,王丹,章杰兵,姜云云,陈云红.HPLC法测定复方紫黄乳膏中盐酸小檗碱的含量[J].解放军药学学报,2013(4):360-361,364. |
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| 作者姓名： | 叶光明 王丹 章杰兵 姜云云 陈云红 |
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| 作者单位： | [1]解放军101医院药剂科,江苏无锡214044 [2]第二炮兵总医院药学部,北京100088 [3]南京军区药品仪器检验所,江苏南京210002 |
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| 基金项目： | 南京军区医学科研课题,No.2011MA012 |
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| 摘 要： | 目的建立HPLC法测定复方紫黄乳膏中盐酸小檗碱的含量。方法采用HPLC法,色谱柱：Agilent EclipseXDB-C18（4.6 mm×250 mm,5μm）,流动相：乙腈-0.05 mol.L-1磷酸二氢钠溶液（磷酸调节pH为3.0）（30∶70）,检测波长265 nm,柱温28℃,流速1 ml.min-1。结果盐酸小檗碱在20～100μg.ml-1范围内浓度与峰面积呈良好的线性关系,回归方程为A=107.81 C-409.19（r=0.9993）,平均回收率为99.53%,RSD为2.14%。结论该方法操作简便,快速易行,结果准确可靠,可作为复方紫黄乳膏的含量测定方法。
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| 关 键 词： | 复方紫黄乳膏 盐酸小檗碱 HPLC |
Determination of Berberine Hydrochloride in Compound Zihuang Cream by HPLC |
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| YE Guang-ming①,WANG Dan②,ZHANG Jie-bing③,JIANG Yun-yun①,CHEN Yun-hong.Determination of Berberine Hydrochloride in Compound Zihuang Cream by HPLC[J].Pharmaceutical Journal of Chinese People's Liberation Army,2013(4):360-361,364. |
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| Authors: | YE Guang-ming① WANG Dan② ZHANG Jie-bing③ JIANG Yun-yun① CHEN Yun-hong |
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| Institution: | ① ①NO.101 Hospital of PLA,Wuxi 214044,China;②General Hospital of Second Artillery,Beijing 100088,China; ③Institute for Inspection of Drug and Instrument of Nanjing Military Region of PLA,Nanjing 210002,China |
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| Abstract: | Objective To establish a method for determination of berberine hydrochloride in compound Zihuang cream by HPLC.Methods The separation was performed on an Agilent Eclipse XDB-C18（4.6 mm × 250 mm,5 μm） column.The mobile phase was acetonitrile-0.05 mol · L-1 NaH2PO4（adjusted to pH 3.0 with phosphoric acid）.The detection wavelength was at 265 nm at a flow rate of 1 ml · min-1 with the column temperature at 28 ℃.Results The linear range of berberine hydrochloride was 20-100 μg · ml-1 and the regression formulation was A=107.81C-409.19（r=0.9993）.The average recovery was 99.53%（RSD=2.14%）.Conclusion This method is simple,accurate,reproducible,and can be used for determination of berberine hydrochloride in compound Zihuang cream. |
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| Keywords: | compound Zihuang cream berberine hydrochloride HPLC |
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