Analysis of the mitochondrial and nuclear genomes of two basidiomycetes,Coprinus cinereus and Coprinus stercorarius |
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Authors: | Christine A. Weber Michael E. S. Hudspeth Gordon P. Moore Lawrence I. Grossman |
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Affiliation: | (1) Department of Cellular and Molecular Biology, Division of Biological Sciences, The University of Michigan, 48109 Ann Arbor, MI, USA;(2) Present address: Biomedical Sciences Division, L-452, Lawrence Livermore National Laboratory, University of California, 94550 Livermore, CA, USA;(3) Present address: Plant Molecular Biology Center, Department of Biological Sciences, Northern Illinois University, 60115-2861 DeKalb, IL, USA;(4) Present address: Gene Expression Group, NEN Products Division, DuPont BRL-500-3, 01862 North Billerica, MA, USA;(5) Present address: Science, 1333 H Street, N. W., 20005 Washington, DC, USA |
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Abstract: | Summary The mitochondrial and nuclear genomes of Coprinus stercorarius and C. cinereus were compared to assess their evolutionary relatedness and to characterize at the molecular level changes that have occurred since they diverged from a common ancestor. The mitochondrial genome of C. stercorarius (91.1 kb) is approximately twice as large as that of C. cinereus (43.3 kb). The pattern of restriction enzyme recognition sites shows both genomes to be circular, but reveals no clear homologies; furthermore, the order of structural genes is different in each species. The C. stercorarius mitochondrial genome contains a region homologous to a probe derived from the yeast mitochondrial var1 gene, whereas its nuclear genome does not. By contrast, the C. cinereus nuclear, but not mitochondrial, genome contains a region homologous to the var1 probe. Only a small fraction of either the nuclear or mitochondrial genomes, perhaps corresponding to the coding sequences, is capable of forming duplexes in interspecies solution reassociations, as measured by binding to hydroxylapatite. Those sequences capable of reassociating were found to have approximately 15% divergence for the mitochondrial genomes and 7%–15% divergence for the nuclear genomes, depending on the conditions of reassociation. |
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Keywords: | Mitochondrial DNA Coprinus DNA-DNA reassociation Gene mapping |
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