| 喉鳞状细胞癌DNA含量的FCM定量分析 |
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| 引用本文： | 杨秋安,钱哨.喉鳞状细胞癌DNA含量的FCM定量分析[J].山东医大基础医学院学报,1999,13(4):198-201. |
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| 作者姓名： | 杨秋安 钱哨 |
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| 作者单位： | 山东医科大学附属医院!250012(杨秋安，赵维萍)，济南妇幼保健院!250001(钱哨) |
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| 摘 要： | 选择２４ 例未经治疗的喉鳞状细胞癌患者术后新鲜肿瘤标本，通过流式细胞仪（ＦＣＭ）测定ＤＮＡ 含量的３ 个主要参数，异倍体（ａｎｅｕｐｌｏｉｄ）、ＤＮＡ 指数（ＤＮＡ Ｉｎｄｅｘ，ＤＩ）和Ｓ期细胞比例（Ｓ－ｐｈａｓｅ ｆｒａｃ－ｔｉｏｎ，ＳＰＦ），探索３ 个参数与肿瘤组织学分级、临床分期以及淋巴结转移之间的关系。结果表明，２４ 例喉鳞状细胞癌中，异倍体检出率为８３．３％ （２０／２４）。ＤＩ与肿瘤组织学分级和淋巴结转移无关，与肿瘤临床分期有关（Ｐ＜ ０．０５）。ＳＰＦ与肿瘤组织学分级无关，与临床分期及淋巴结转移有关（Ｐ＜ ０．０１，Ｐ＜ ０．０５）。提示：（１）ＤＮＡ异倍体是喉鳞状细胞癌的重要特征之一；（２）ＤＩ和ＳＰＦ可作为预测喉鳞状细胞癌生物学特性的重要指标
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| 关 键 词： | 喉肿瘤 流式细胞术 DNA倍体 DNA指数 S期细胞比例 |
THE QUANTITATIVE ANALYSIS OF DNA CONTENTS IN SQUAMOUS CARCINOMA OF LARYNX USING FCM |
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| Abstract: | Fresh specimens from 24 previously untreated patients with squamous carcinomas of larynx(LSCC)were selected.Aneuploid,DNA index(DI)and S-phase fraction(SPF)were measured using flow cytometry(FCM).The association between these parameters with histologic grades,clinical stage and lymph node metastasis were studied.The results showed that the ratio of aneuploid was 83.3%(20/24).DI was not correlated with histologic grades and lymph node metastases,but correlated with clinical stages (P<0.05).SPF was correlated both with clinical stages(P<0 01)and lymph node metastasis(P<0 05) SPF had no relation with histologic grades.It is concluded that DNA aneuploid is one of the most important specialities in LSCC,meanwhile DI SPF can be regarded as significant predictors of biologic characteristics in LSCC. |
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| Keywords: | Larynx neoplasms Flow cytometry DNA ploid DNA index S-phase fraction |
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