| 钙化性主动脉瓣疾病瓣膜间质细胞的生物学特征分析 |
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| 引用本文： | 刘延玲,刘晓红,龚德军,袁扬,陶婧,黄盛东,韩林. 钙化性主动脉瓣疾病瓣膜间质细胞的生物学特征分析[J]. 第二军医大学学报, 2011, 32(6): 617-621. DOI: 10.3724/SP.J.1008.2011.00617 |
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| 作者姓名： | 刘延玲 刘晓红 龚德军 袁扬 陶婧 黄盛东 韩林 |
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| 作者单位： | 第二军医大学长海医院胸心外科,上海 200433 |
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| 基金项目： | 上海市科委科技基金(2R1438000). |
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| 摘 要： | 目的初步分析钙化性主动脉瓣疾病(calcific aortic valve disease,CAVD)瓣膜间质细胞的生物学特征,为后续研究奠定基础。方法采用组织块接种和免疫磁珠法分选正常主动脉瓣和CAVD瓣膜间质细胞,观察其形态学和行为学特征。通过免疫细胞化学染色和流式细胞术分析CAVD瓣膜间质细胞免疫表型的改变。结果与正常主动脉瓣瓣膜间质细胞相比,CAVD瓣膜间质细胞呈肌纤维母细胞和成骨细胞形态,当细胞达到一定密度后细胞自发性收缩、聚集样生长并形成钙化结节。体外培养的CAVD瓣膜间质细胞表达肌纤维母细胞标志物α-SMA和成骨细胞标志物碱性磷酸酶(ALP)。结论 CAVD瓣膜间质细胞生物学特征的改变可能是导致主动脉瓣增厚、钙化和交界融合的重要因素。
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| 关 键 词： | 钙化性主动脉瓣疾病 瓣膜间质细胞 α-平滑肌动蛋白 碱性磷酸酶 成骨细胞 |
| 收稿时间： | 2011-03-24 |
| 修稿时间： | 2011-06-06 |
Biological characteristics of valvular interstitial cells in calcific aortic valve disease |
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| LIU Yan-ling,LIU Xiao-hong,GONG De-jun,YUAN Yang,TAO Jing,HUANG Sheng-dong and HAN Lin. Biological characteristics of valvular interstitial cells in calcific aortic valve disease[J]. Former Academic Journal of Second Military Medical University, 2011, 32(6): 617-621. DOI: 10.3724/SP.J.1008.2011.00617 |
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| Authors: | LIU Yan-ling LIU Xiao-hong GONG De-jun YUAN Yang TAO Jing HUANG Sheng-dong HAN Lin |
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| Affiliation: | Department of Cardiothoracic Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China |
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| Abstract: | Objective To observe the biological characteristics of valvular interstitial cells in calcific aortic valve disease(CAVD),so as to lay a foundation for future study.Methods Tissue culture inoculation and immunomagnetic bead separation method were used to isolate the valvular interstitial cells from the normal aortic valves and CAVD valves.The morphological and behavioral characteristics of the isolated valvular interstitial cells were observed.Immunocytochemistry and flow cytometry analysis were employed to... |
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