通络方剂对1型糖尿病大鼠糖脂代谢的影响

目的探讨通络方剂对1型糖尿病大鼠糖脂代谢的影响。方法 30只成年雄性SD大鼠随机分为正常对照组(NC)、糖尿病组(DM)、通络方剂治疗组(TLR),每组10只;后两组大鼠采用链脲佐菌素(STZ)60 mg/kg腹腔注射制备1型糖尿病模型。模型制备成功后,通络方剂组给予通络方剂(1.0 g.kg-1.d-1)灌胃,糖尿病组、正常对照组给予同剂量双蒸水灌胃。8周后比较各组大鼠体质量、附睾周围脂肪组织质量、空腹血糖(FBG)、空腹胰岛素(FINS)及血总胆固醇(TC)、三酰甘油(TG)、游离脂肪酸(FFA)、瘦素(leptin)水平,检测脂肪组织中瘦素mRNA表达水平。结果与糖尿病组相比,通络方剂治疗组大鼠体质量增加(P<0.01)、血FBG降低(P<0.05)、FINS升高(P<0.01)、FFA降低(P<0.01)、血leptin水平增加(P<0.05);脂肪组织leptin mRNA表达水平升高(P<0.05)。结论通络方剂可能通过改善糖尿病大鼠瘦素、胰岛素低水平状态纠正糖脂代谢紊乱。

Effect of Tongluo recipe on leptin and insulin in rat model of type 1 diabetes mellitus

ObjectiveTo study the effect of Tongluo recipe on leptin and insulin in rat model of type 1 diabetes mellitus.MethodsA total of 30 adult male SD rats were equally randomized into the following 3 groups: normal control group, diabetes mellitus group and Tongluo recipe group. SD rats were administered intraperitoneally with STZ(60 mg/kg) to induce the model of type 1 diabetes mellitus. The rats in Tongluo recipe group were administered intragastrically with Tongluo recipe(1.0 g·kg-1·d-1), and the other two groups were administered intragastrically with double distilled water in the same dosage. The body weight, white adipose tissue weight, fasting blood glucose, fasting insulin, total cholesterol, triglyceride, free fatty acid, and leptin levels were compared between each group 8 weeks later. The expression of leptin mRNA in white adipose tissue was examined. ResultsCompared with diabetes mellitus group, the body weight, fasting insulin, and leptin were significantly higher (P<0.05 or 0.01) in the Tongluo recipe group, the levels of fasting blood glucose, total cholesterol, and free fatty acid were significantly lower(P<0.05 or 0.01) in Tongluo recipe group, and the expression of leptin mRNA was significantly higher (P<0.05) in the Tongluo recipe group. ConclusionTongluo recipe may correct the disturbance in the lipid and glucose metabolism by improving the low leptin and insulin levels in diabetic rats.