环氧合酶-2抑制剂塞来昔布对早期糖尿病大鼠视网膜组织促红细胞生成素的影响

目的：观察塞来昔布环氧合酶-2（ COX-2）选择性抑制剂引起糖尿病大鼠视网膜组织促红细胞生成素的改变，探讨其防治早期糖尿病视网膜的机制。方法40只SD雄性大鼠随机分为4组，即正常组、糖尿病组、对照组、实验组，每组10只。1％链尿佐菌素腹腔注射建立糖尿病模型，饲养3个月后，处死大鼠取视网膜，HE染色观察视网膜形态学变化，SYBR荧光实时定量PCR检测视网膜组织中COX-2、EPO mRNA表达变化。结果3个月后，HE染色糖尿病组和对照组结构出现了改变；COX-2、EPO mRNA在糖尿病组和对照组，组间两两比较，无统计学差异（ P ＜0．05），其余各组间比较均有显著性差异（ P ＞0．05）。结论塞来昔布能够有效促进EPO的表达，可以用于早期糖尿病视网膜病变防治。

Effect of celecoxib,a cyclooxygenase-2 inhibitor,on the expression of erythropoietin in retina of diabetic rats

Objective To observe the effect of cyclooxygenase-2 inhibitor on the expression of erythropoietin （ EPO） in diabetic rats retina and the effect of celecoxib on prevention of early diabetic retinopathy .Methods Forty SD male rats were randomly divided into four groups：the normal group , diabetes group , control group and experimental group , 10 in each group.Diabetes model was established by intraperitoneal injection of streptozotocin （STZ） （50 mg/kg）.All of the rats were sacrificed at 3 months.Retinae were HE stained to observe morphological changes , and SYBR real-time fluo-rescent quantitative PCR was used to detect COX-2 and EPO mRNA expression changes .Results After 3 months, HE staining showed structural changes in diabetes group and control group .There was no difference in COX-2 or EPO mRNA expression between diabetes group and the control group （ P 〈0.05）, but between diabetes group and other two groups （ P 〈0.05）.Conclusions Celecoxib could effectively promote the expression of EPO , thus can be used for early preven-tion and treatment for diabetic retinopathy .