急性白血病P16基因的研究

目的：研究Ｐ１６抑癌基因在急性白血病中的变化。方法：用ＡＢＣ法研究了６１例急性白血病细胞表面Ｐ１６抗原的多表达多重ＰＣＲ法，研究了５１例急性白血病Ｐ１６基因的结构缺陷。结果：发现白血病患者Ｐ１６抗原的表达明显低于正常人（Ｐ〈０．００１），其中急性淋巴细胞白血病（ＡＬＬ）又明显低于急性髓系白血病（ＡＭＬ）（Ｐ〈０．０５）；３０例ＡＬＬ中仅发现４例Ｐ１６基因第二外显子纯合子缺失，２１例ＡＭＬ未发现Ｐ１

Study on P16 Gene in Acute Leukemia

To study on change of suppressing cancer gene P16 in acute leukemia,the antgen expresion of P16 in 61 cases of leukemic cell surfaces with ABC assay and gene structure defect in 51 cases of acute leukemia with multiple comparative PCR method were investigated. It was found that antigen expresion of P16 in leukemia was obviously low than that of in normal people (P <0.001). At the same time,antigen expresion in ALL was low than that of in AML (P<0.05). The exon 2 of P16 gene showed homozyous deletion only in 4 cases out of 30 cases in ALL. There was no structure defect in 21 cases of AML. It was demonstrated that expression defect of P16 gene was main cause in development, evolution of acute leukemia, and structure defect was not main molecular event.