| 卡氏肺孢子虫标本染色方法的改进 |
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| 引用本文： | 王元松,常志尚,张忠广,曾宪忠,宫玉香.卡氏肺孢子虫标本染色方法的改进[J].青岛大学医学院学报,2004,40(3):278-279. |
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| 作者姓名： | 王元松 常志尚 张忠广 曾宪忠 宫玉香 |
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| 作者单位： | 青岛大学医学院人体寄生虫学教研室,山东,青岛,266021 |
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| 基金项目： | 山东省科技厅科研基金资助项目(02-1-kj-yn-43) |
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| 摘 要： | ①目的改进卡氏肺孢子虫标本的染色方法，以便准确诊断卡氏肺孢子虫肺炎和制作教学标本。②方法采用吉姆萨-瑞特染色法。③结果染色后卡氏肺孢子虫包囊囊内小体细胞核呈紫红色，细胞质呈蓝色，囊壁呈淡蓝色，各部分结构清晰可辨。滋养体包核为紫红色，胞质为蓝色。④结论吉姆萨-瑞特染色法操作简便、快速，卡氏肺孢子虫标本着色清晰，是临床诊断和教学中值得推广和应用的染色方法。
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| 关 键 词： | 肺囊虫 卡氏 染色与标记 诊断技术和方法 |
| 文章编号： | 1672-4488(2004)03-0278-02 |
| 修稿时间： | 2003年12月1日 |
AN IMPROVED METHOD FOR STAINING PNEUMOCYSTIS CARINII |
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| WANG Yuan song,CHANG Zhi shang,ZHANG Zhong guang,et al.AN IMPROVED METHOD FOR STAINING PNEUMOCYSTIS CARINII[J].Acta Academiae Medicinae Qingdao Universitatis,2004,40(3):278-279. |
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| Authors: | WANG Yuan song CHANG Zhi shang ZHANG Zhong guang |
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| Abstract: | Objective To improve the staining of Pneumocystis Carinii specimen for a correct diagnosis of pneumocystis carinii pneumonia(PCP) and preparation of teaching specimen. Methods The specimens were stained with Wright Giemsa compound staining. Results After the staining, the structures of pneumocystis carrinii cyst were seen clearly, with amaranth cell of intracystic body nucleus, blue cytoplasm, light blue cyst walls. The nucleus and cytoplasm of trophozoite was amaranth and blue, respectively. Conclusion Wright Giemsa compound staining is easy and quick,with bright and clear specimens,which is worth of clinical application and teaching. |
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| Keywords: | pneumocystis carinii staining and labeling diagnostic techniques and procedures |
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