氯沙坦对自发性高血压大鼠左心室肥厚和转化生长因子β_1的影响

目的 :探讨转化生长因子 β1 (TGFβ1 )在自发性高血压大鼠(SHR)左心室肥厚中的作用及氯沙坦对左心室肥厚和TGFβ1 水平的影响。方法 :2 0只SHR随机分成氯沙坦治疗组和未治疗组 ,每组10只 ,用免疫组织化学法检测SHR心肌TGFβ1 的表达及氯沙坦治疗后的改变 ,同时观察心肌肥厚的变化 ,并与正常WKY组对照。结果 :正常组心肌细胞胞浆内TGFβ1 表达呈弱阳性 ,SHR对照组呈强阳性 ,氯沙坦治疗组呈阳性反应。用图像分析仪计算其平均吸光度分别为 17.46± 2 .3 8、15 6.81± 2 1.75、67.19± 7.2 4,并且SHR左心室质量指数 (LVWI)高于WKY组 ,氯沙坦治疗组LVWI低于对照组。结论 :TGFβ1 在自发性高血压大鼠心肌中表达增强 ,而氯沙坦治疗可抑制其表达 ,从而可能延缓SHR左心室肥厚的病理学进展。

Effect of Losartan on Left Ventricular Hypertrophy and the Level of Transforming Growth Factorβ1 in Spontaneously Hypertensive Rats

Objective: To study the role of plasma TGFβ_1 in left ventricular hypertrophy (LVH) and effect of losartan on LVH and the level of TGFβ_1 in spontaneously hypertensive rats(SHR).Method: 20 SHR were divided randomly into two group :Losartan group( n =10) and SHR group( n =10). The expression of TGF-β_1 was detected by immunohistochemical staining and the left ventricular hypertrophy was examined in the two groups,and compared with the control group.Results: The expression of TGFβ_1 is significantly higher in SHR than WKY rat,after 8 weeks treated with Losartan,the level of TGFβ_1 and LVMI are decreased.Conclusion: TGFβ_1 has an important role in LVH,Losartan could reduced the level of TGFβ_1 in left ventricular and LVWI.