ATP抑制不死化人成纤维细胞增殖信号转导机制的研究

目的:探讨ATP抑制不死化人成纤维细胞增殖信号转导机制。方法:观察0.4mmol/LATP与不死化人成纤维细胞共培养不同时间下,细胞内游离Ca²⁺浓度(Ca²⁺]i)和三磷酸肌醇(IP₃)浓度的变化特点和意义,以及分别应用Ca²⁺和K⁺通道拮抗剂时,细胞增殖率的改变。结果:ATP与不死化人成纤维细胞共培养时,Ca²⁺]i明显升高,IP₃浓度明显降低(均P＜0.01)。分别应用Ca²⁺和K⁺通道拮抗剂时,细胞增殖率都显著增高(均P＜0.01)。结论:ATP抑制不死化人成纤维细胞增殖过程中有钙通道和钾通道以及IP₃的参与。

Inhibitory effect of ATP on proliferative signaling in immortalized human fibroblasts

AIM: To investigate the inhibitory effects of ATP on proliferation signaling in immortalized human fibroblasts. METHODS: Immortalized human fibroblasts were treated with ATP, ATP conbined with calcium or potassium channel antagonists, respectively. The intracelluar-free calcium (Ca 2 ]i), inositol 1,4,5-trisphosphate(IP 3) levels and cell viability were detected at different time points. RESULTS: ATP significantly increased the Ca 2 ]i and decreased the IP 3 level in immortalized human fibroblasts, especially at initial stage ( P< 0 01) . Compared to ATP alone, the proliferation rates remarkably increased when calcium or potassium channel antagonists were used ( P< 0 01, respectively) with ATP. CONCLUSION: The calcium and potassium channels and IP 3 involved in the inhibitory effects of ATP on the proliferative signaling in immortalized human fibroblasts.