| 脑室注射γ-氨基丁酸对雄性大鼠下丘脑促性腺激素释放激素含量的影响 |
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| 引用本文： | 曹兴福,马虹宇,王京花,张丽敏,马青年.脑室注射γ-氨基丁酸对雄性大鼠下丘脑促性腺激素释放激素含量的影响[J].生殖医学杂志,1997(4). |
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| 作者姓名： | 曹兴福 马虹宇 王京花 张丽敏 马青年 |
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| 作者单位： | 哈尔滨医科大学生理教研室，黑龙江省医院实验诊断部 |
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| 摘 要： | 本研究观察了不同剂量γ－氨基丁酸（ＧＡＢＡ）对雄性大鼠下丘脑促性腺激素释放激素（ＧｎＲＨ）含量的作用。结果表明，脑室注射５、５０、２５０及５００ｍｍｏｌ／ＬＧＡＢＡ各为２０μｌ，４０分钟后下丘脑ＧｎＲＨ含量分别为３．５７±０．５８、３．７５±０．６６、４．６３±０．６３及５．０７±０．５９ｎｇ／１０ｍｇ湿重组织，均显著高于对照组下丘脑ＧｎＲＨ含量（１．７６±０．２１ｎｇ／１０ｍｇ湿重组织，Ｐ＜０．０５）。脑室注射２５０ｍｍｏｌ／ＬＧＡＢＡ２０μｌ后２０、４０、６０、１２０及２４０分钟下丘脑ＧｎＲＨ含量依次为２．２４±０．２９、４．６３±０．６３、５．１１±０．３８、４．２０±０．４０及３．９８±０．９１ｎｇ／１０ｍｇ湿重组织。ＧＡＢＡ受体拮抗剂荷包牡丹碱（ｂｉｃｕｃｕｌｉｎｅ，Ｂｉｃ）可阻断ＧＡＢＡ对下丘脑ＧｎＲＨ含量的促进作用。
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| 关 键 词： | 脑室 γ-氨基丁酸 促性腺激素释放激素 下丘脑 |
Effect of intracerebroventricular injection of GABA on hypothalamic gonadotrophin releasing hormone content in male rats |
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| Abstract: | Objective: To investigate the effect of intracerebroventricular injection of GABA on hypothalamic gonadotrophin releasing hormone(GnRH) content in male rats.Design: An experimental animal study.Setting: Harbin Medical University.Methods: Male rats were anaesthetized with ether,then the saline (20μl) and GABA solution (at 5、50、250 and 500 mmol/L,20μl) were injected intracerebroventricularly.The hypothalamic tissues were removed at 40 min after intracerebroventricular injection of GABA,followed by homogenization and centrifugation.The GnRH content in supernates of hypothalamic homogenates were measured by RIA.Results: The hypothalamic GnRH contents in rats were 3.57±0.58,3.75±0.66,4.63±0.63 and 5.07±0.59 ng/10 mg wet tissue at 40 min after intracerebroventricular injection of 5、50、250 and 500 mmol/L GABA 20 μl respectively which were significantly higher than those in control group (1.76±0.21 ng/10 mg wet tissue,P<0.05). The effect seems to be dose dependent and it can be blocked by bicuculline.Conclusion: The results indicated that GABA can stimulate GnRH production in the hypothalamus of male rat. |
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| Keywords: | Cerebral ventricles GABA Gonadotrophin releasing hormone Hypothalamus |
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