口腔癌中诱导型一氧化氮合酶与血管内皮生长因子表达关系的研究

目的研究诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)在口腔癌中表达水平以及与口腔癌血管内皮生长因子(vascular endothelial growth factor,VEGF)表达和淋巴结转移的关系.方法以正常口腔粘膜作对照,采用免疫组化SP法检测41例口腔癌组织中iNOS的表达,同时检测口腔癌中VEGF的表达和微血管密度(MVD)(CD34标记).结果口腔癌组织iNOS阳性表达率为63.41%,淋巴结阴性组和阳性组差异有显著性(P<0.01),而9例正常口腔粘膜表达均为阴性;在iNOS不同表达组间MVD值差异有高度显著性(P<0.001),VEGF的表达差异有显著性(P<0.05).结论 iNOS在口腔癌中高表达,并与肿瘤血管形成、淋巴结转移密切相关;它可能参与VEGF促血管生成作用.

Relationship between expression of inducible nitric oxide synthase and of VEGF in oral carcinoma

OBJECTIVE: To study the expression of inducible NOS in oral carcinoma and it's relationship with the expression of VEGF, MVD and lymphatic metastasis. METHODS: With 9 cases of normal oral mucosa as control, the expression of iNOS in 41 cases of oral carcinoma was evaluated by immunohistochemistry SP staining. Meanwhile MVD and the expression of VEGF were also detected. RESULTS: The expression rate of iNOS in 41 cases was 63.41%, and the rate between N+ group and N- group was significantly different (P < 0.01). There were significant difference of MVD (P < 0.001) and of the expression of VEGF(P < 0.05) among groups of different iNOS expression level. CONCLUSIONS: The expression of iNOS in oral carcinomas is high, and it is closely related to angiogenesis and lymphatic metastasis. The expression of iNOS probably takes part in VEGF's angiogenic effect.