67例结肠癌DNA倍体和Ag—NOR计数分析对判断患者预后价值的初步探讨

对６７例结肠癌石蜡包埋组织材料做ＤＮＡ倍体水平分析和Ａｇ—ＮＯＲ计数。结果见：ＤＮＡ二倍体肿瘤５年、７年生存率分别为７８．０％（３２／４１）、３９．０％（１６／４１）；非二倍体肿瘤则为２６．９％（７／２６）、１１．５％（３／２６）（ｐ＜０．０１、ｐ＜０．０５）；Ａｇ─ＮＯＲ计数小于均值（６．９±１．０）组５年、７年生存率为８０．０％（２８／３５）、４０．０（１４／３５），大于均值组则为３４．４％（１１／３２）、１５．６％（５／３２）（ｐ＜０．０１、ｐ＜０．０５）。Ａｇ－ＮＯＲ计数小于均值组ＤＮＡ二倍体肿瘤占９７．１％（３４／３５）；大于均值组二倍体肿瘤占２１．９％（７／３２）（ｐ＜０．０１）。结果表明：ＤＮＡ倍体水平和Ａｇ－ＮＯＲ计数具有一致性，可以做为判断结肠癌预后指标。

A preliminary study of DNA ploidy and Ag-NOR counts on the prognostic evaluation of 67 patients with colonic cancer

Flow cytometry(FCM) and silver colloid staining technique have special valve for retrospective analyses of the tumors.The DNA ploidy and Ag-NOR counts in 67 paraffin-embeded samples were analyzed,5 or 7-year survival rate was 78.0%(32/41) and 39.0%(16/41) for the patients with DNA diploid tumor,while 26.9%(7/26) and 11.5%(3/26) for the patients with DNA non-diploid trmpr(p<0.O1 and p<0.05 respectively).The survival percentage at 5 or 7 yearswas 80.0%(28/35) and 40.0%(14/35) for the patients with Ag-NOR counts less thaw X±SD=6.9±1.O,while 34.4(11/32) and 15.6%(5/32) for those with Ag-NOR counts more than that(p<0.0l and p<0.05 respectively).DNA ploidy and Ag-NOR counts were closely related to prognostic factors of colonic cancer.DNA diploid was 97.7%(34/35) for the patients with Ag-NOR counts less than the mean of 6.9±1.O,while 21.9%(7/32) for the patients with Ag-NOR counts more than that(p<0.01).These findings suggest that DNA ploidy and Ag-NOR counts be considered as prognostic parameters of colonic cancer.