糖尿病小鼠模型的建立及中药舒糖宝疗效研究

目的探讨糖尿病动物模型的建立及中药舒糖宝的治疗效果。方法小鼠分成4组:正常组、模型组、治疗1组、治疗2组,后3组建立糖尿病小鼠模型,用四氧嘧啶按200mg/kg质量给小鼠腹腔注射1次后,相隔3 d,再用链佐霉素按40 mg/kg体质量连续注射4 d,每天1次,血糖升高后,治疗1组和治疗2组分别用舒糖宝和盐酸二甲双胍灌胃治疗。测定造模前、造模后、治疗后血糖含量。实验结束,从眼球取血,测定总胆固醇(TC)、三酰甘油(TG)含量。结果正常组、模型组、治疗1组、治疗2组血糖含量,造模后2 d分别为(6.25±1.21),(13.72±3.87),(18.54±9.83),(15.86±6.17)mmol/L,后3组与正常组比较有显著性差异(P<0.05或0.01);造模后5 d血糖分别为(5.74±1.19),(17.03±10.22),(19.67±4.97),(17.09±5.51)mmol/L,后3组与正常组比较有显著性差异(P均<0.01);治疗24 d血糖分别为(6.99±0.92),(17.14±8.69),(16.79±7.00),(10.99±2.24)mmol/L,模型组未降低,治疗2组降低明显,治疗1组也有一定程度降低。TC、TG各组间比较无明显差异。结论中药舒糖宝降糖疗效优于盐酸二钾双胍。

Study on establishment of diabetic model in mice and traditional Chinese medicine treatment effect of Shutangbao

Objective It is to approach the establishment of the animal model of diabetes mellitus and observe the curative effect of traditional Chinese medicine Shutangbao.Methods All mice were divided into four groups: normal group,model group,treatment group 1 and treatment group 2.In last 3 groups,the diabetic mouse model was established with intraperitoneal injection of Alloxan according to 200 mg/kg body weight dose once,after 3 days apart,with intraperitoneal injection of STZ according to 40 mg/kg body weight for 4 days,once a day.When the FPG was increased the mice in treatment group 1 and treatment group 2 accepted intragastric administrations with Shutangbao and dianoguanil respectively.The blood glucose levels were detected before making model,after establishment of model,after treatment.The end of the experiment,blood was collected from the eye to detect the content of total cholesterol(TC) and triglyceride(TG).Results In normal group,model group,treatment group 1 and treatment group 2,the blood glucose levels was(6.25± 1.21),(13.72±3.87),(18.54±9.83),(15.86±6.17) mmol/L respectively after modeling for 2 days,there were significant differences between the last 3 groups and normal group(P<0.05 or 0.01);and was(5.74±1.19),(17.03±10.22),(19.67±4.97),(17.09±5.51) mmol/L respectively after modeling for 5 days,there were very significant differences between them(all P<0.01);and was(6.99±0.92),(17.14±8.69),(16.79±7.00),(10.99±2.24) mmol/L respectively after treatment for 24 days,the level in treatment group 2 was reduced obviously,and had a certain degree to reduce in treatment group 1 also.There was no significant difference in levels of TC and TG among all groups.Conclusion The curative effect of Shutangbao for diabetes mellitus is better than that of dianoguanil.