整合素α5β1与大鼠肝纤维化发生的实验研究

目的]肝星状细胞（HSC）活化是肝纤维化的病理基础，本研究旨在探讨整合素受体在HSC活化及肝纤维化过程中的作用。方法]制作大鼠肝硬化模型，同时进行体外细胞株HSC—T6实验。用免疫组化法观察仅平滑肌肌动蛋白（α-SMA）、整合素α5β1及纤维连接蛋白（FN）的表达。HSC细胞株在FN包被的皿上培养，测定细胞活化、α-SMA、整合素α5β1蛋白表达。结果]（1）模型组肝组织FN、α-SMA和α5β1蛋白表达增多。（2）FN包被明星促进HSC—T6的活化、α—SMA及α5β1的表达。结论]FN促进整合素α5β1表达是活化HSC、促进肝纤维化的重要途径。

Study of integrin α5β1 on development of rat hepatic fibrosis

Objective] To investigate the variation of fibronectin（ FN）, integrin α5β1 and hepatic stellate cells（HSC） activation during liver fibrogenesis. Methods ] Model of rat liver fibrosis and HSC cell line （ HSC - T6） were employed. The expression of α -SMA, FN and integrin α5β1 protein in liver tissue were assayed. HSC -T6 cell line was cultured on uncoated or FN - coated plastic dishes. HSC activation, the expression of α - SMA and integrin α5β1 protein in HSC were determined. Results] A high expressions of FN, α5β1 and α - SMA were observed in model group. FN increased α - SMA, and α5β1 expression of HSC -T6. Conclusion] Increasing expression of FN and integrin α5β1 is a important machanism for activation of HSC, during development of liver fibrosis.