| 环介导等温扩增技术及在传染性疾病检测中的应用 |
| |
| 引用本文： | 蔡哲钧,冯杰雄. 环介导等温扩增技术及在传染性疾病检测中的应用[J]. 国际流行病学传染病学杂志, 2006, 33(5): 343-345,358 |
| |
| 作者姓名： | 蔡哲钧 冯杰雄 |
| |
| 作者单位： | 430074,武汉,华中科技大学同济医学院;430030,武汉,华中科技大学同济医院 |
| |
| 摘 要： | 介绍环介导等温扩增技术的的基本原理、特点及在传染性疾病中检测和诊断的应用。环介导等温扩增技术具有简单、快速、特异性强和扩增效率高等的特点,目前可检测HBV、流感病毒、疱疹病毒、水痘_带状疱疹病毒、腮腺炎病毒、麻疹病毒、腺病毒、SARS病毒、呼吸道合胞病毒、西尼罗病毒、结核分枝杆菌、痢疾志贺菌、大肠埃希菌、螺旋体、肺炎链球菌和耶氏菌等。
|
| 关 键 词： | 核酸 环介导等温扩增 传染病 检测 |
| 收稿时间： | 2006-05-05 |
| 修稿时间： | 2006-05-05 |
Loop-mediated isothermal amplification method for the diagnosis of infectious diseases |
| |
| CAI Zhe-jun,FENG Jie-xiong. Loop-mediated isothermal amplification method for the diagnosis of infectious diseases[J]. International Journal of Epidemiology and Infectious Disease, 2006, 33(5): 343-345,358 |
| |
| Authors: | CAI Zhe-jun FENG Jie-xiong |
| |
| Affiliation: | Torsi Medical College of Huazhoug University of Science and Technology, Wuhan 430074, China |
| |
| Abstract: | Loop-mediated isothermal amplification(LAMP) is a novel nucleic acid amplification method in which reagents react with high specificity,efficiency and speed,and therefore has great prospect for the diagnosis of infectious deceases.Here we reviewed the principle and application of LAMP.It has the ability to detect HBV,influenza A viruses,herpes simplex virus,varicella-zoster virus,mumps virus,measles virus,adenovirus,severe acute respiratory syndrome coronavirus,respiratory syncytial virus,West Nile virus,Mycobacterium tuberculosis,Shigella,Escherichia coli,Streptococcus pneumoniae and Yersinia enterocolitica,etc. |
| |
| Keywords: | Nucleic acids Loop-mediated isothermal amplification Infectious diseases Detection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
