| Abstract: | Light microscopy study of direct smear of cells
cultured in vitro revealed definite cellular atypia,
abnormal mitoses and gland-like arrangement of
the cells. In cells of the original human gastric
carcinoma specimen, PAS positive secretory granules
were present, but no AB positive ones. Tumor
tissue obtained after animal inoculation showed the
presence of glandular structure with HE stain, and
nest-like structure was demonstrated electronmicro-
scopically. Also revealed were a few "A cells"
secretory granules as well as junctional complexes
characteristics of epithelial tissue. In addition,
Leuchtenberger's inclusion bodies were found
within the in vitro cultured cells. This also support-
ed their epithelial origin and malignant nature. The
original human gastric carcinoma was differentiated
adenocarcinoma, which manifested with dedifferen
tiation after prolonged culture in vitro. It might
be concluded that the in vitro cultured cells assayed
were adenocarcinomas, and, when considered with
the clinical data, gastric adenocarcinoma. |
