| 喉癌p16、p15基因纯合性缺失的研究 |
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| 引用本文： | 李福才,康宁,李英慧,贺光,林长坤,高宏明,孙兴和,孙开来. 喉癌p16、p15基因纯合性缺失的研究[J]. 中华医学遗传学杂志, 2002, 19(1): 30-32 |
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| 作者姓名： | 李福才 康宁 李英慧 贺光 林长坤 高宏明 孙兴和 孙开来 |
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| 作者单位： | 1. 中国医科大学医学遗传学教研室沈阳,110001
2. 空军463医院耳鼻喉科 |
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| 基金项目： | 国家自然科学基金 (39770 794)，辽宁省自然科学基金(972 2 2 5)~~ |
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| 摘 要： | 目的 研究p16、p15基因缺失状态与喉癌发生、发展的关系。方法 提取喉癌新鲜组织中基因组DNA，采用聚合酶链反应技术，分别对80例喉癌进行p16基因第2外显子（p16E2）和67例喉癌进行p15基因第2外显子(p15E2）纯合性缺失研究。结果 检测p16E2弛合性缺失率为12.5%(10/80)，p15E2纯合性缺失率为11.94%（8／67），p16E2、p15E2共同缺失率为5.97%（4／67）。结论 p16E2和p15E2纯合缺失以及二者共同缺失与喉癌发生有相关性，可能在喉癌发生及恶性进展中起一定作用。
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| 关 键 词： | 喉鳞状细胞瘤 p16基因 p15基因 聚合酶链反应 纯合性缺失 |
| 修稿时间： | 2001-03-07 |
Hommzygous deletion of p16 and p15 genes in laryngeal squamous cell carcinoma |
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| LI Fucai ,KANG Ning ,LI Yinghui ,HE Guang ,LIN Changkun ,SUN Xinghe ,GAO Hongming ,SUN Kailai .. Hommzygous deletion of p16 and p15 genes in laryngeal squamous cell carcinoma[J]. Chinese journal of medical genetics, 2002, 19(1): 30-32 |
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| Authors: | LI Fucai KANG Ning LI Yinghui HE Guang LIN Changkun SUN Xinghe GAO Hongming SUN Kailai . |
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| Affiliation: | Department of Medical Genetics, China Medical University, Shenyang, Liaoning, 110001 P. R. China. Fucaili@hotmail.com |
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| Abstract: | Objective To assess the relationship of homozygous deletion status of p16 (MTS1/ INK4a/ CDKN2A), p15(MTS2/INK4b/CDKN2B) genes and laryngeal squamous cell carcinoma(LSCC) progression. Methods DNA was extracted from fresh tumors. Homozygous deletion of p16 exon 2(p16E2) in 80 cases of LSCC and p15 exon 2(p15E2) in 67 cases of LSCC were detected by the polymerase chain reaction technique. Results The p16E2 deletion rate in 80 cases was 12.5%(10/80); the p15E2 deletion rate in 67 cases was 11.94%(8/67); the p16E2 and p15E2 codeletion rate in 67 cases was 5.97%(4/67). Conclusion Homozygous deletion of p16E2 and p15E2 is related with LSCC oncogenesis, and it may play a role to some extent in LSCC malignant progression. |
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| Keywords: | laryngeal squamous cell carcinoma p16 gene p15 gene polymerase chain reaction homozygous deletion |
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