| 基于指纹图谱和OPLS-DA的越南和国产土茯苓差异性化合物探索 |
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| 引用本文： | 郭威,孙蓉,王亮,詹志来,金艳,杨滨,黄璐琦.基于指纹图谱和OPLS-DA的越南和国产土茯苓差异性化合物探索[J].中国实验方剂学杂志,2017,23(14):62-67. |
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| 作者姓名： | 郭威 孙蓉 王亮 詹志来 金艳 杨滨 黄璐琦 |
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| 作者单位： | 山东中医药大学 药学院, 济南 250355;中国中医科学院 中药研究所, 北京 100700;中国中医科学院 中药资源中心道地药材国家重点实验室培育基地, 北京 100700;山东省中医药研究院, 济南 250014,山东省中医药研究院, 济南 250014,山东中医药大学 药学院, 济南 250355;山东省中医药研究院, 济南 250014,中国中医科学院 中药资源中心道地药材国家重点实验室培育基地, 北京 100700,中国中医科学院 中药资源中心道地药材国家重点实验室培育基地, 北京 100700,中国中医科学院 中药研究所, 北京 100700,中国中医科学院 中药资源中心道地药材国家重点实验室培育基地, 北京 100700 |
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| 基金项目： | 中医药行业科研专项（201407003） |
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| 摘 要： | 目的:分析越南和国产土茯苓化学成分的差异,对差异性化合物进行辨识,并对样品进行产地鉴别。方法:收集越南土茯苓7份,国产土茯苓28份。建立越南和国产土茯苓化学成分色谱指纹图谱,根据相似度评价结果比较两者的差异程度,采用HPLC-MS对指纹图谱的主要色谱峰进行归属,共鉴定21个峰。采用聚类分析对样品进行产地鉴别;结合正交信号校正偏最小二乘判别分析(OPLS-DA)进行差异性化合物的筛选,选取位于S-plot图两端并且VIP1的点作为区分越南与国产土茯苓的差异性化合物。结果:越南和国产土茯苓主要化学成分的含量存在差异性,聚类分析可将35批土茯苓药材分为越南和国产2组,两者的差异性化合物分别为落新妇苷、异落新妇苷、新落新妇苷、黄杞苷,越南土茯苓中差异性化合物的含量显著大于国产土茯苓。结论:采用OPLS-DA分析样品的指纹图谱,既可从整体角度分析越南和国内土茯苓化学成分的异同,又可对两者差异性化合物进行辨识,为越南土茯苓入药的可能性及其质量评价提供实验数据。
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| 关 键 词： | 土茯苓 指纹图谱 校正偏最小二乘判别分析 差异性化合物 |
| 收稿时间： | 2017/2/23 0:00:00 |
Discrimination Markers of Smilacis Glabrae Rhizome from Vietnam and China Based on Fingerprint Combined with OPLS-DA |
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| GUO Wei,SUN Rong,WANG Liang,ZHAN Zhi-lai,JIN Yan,YANG Bin and HUANG Lu-qi.Discrimination Markers of Smilacis Glabrae Rhizome from Vietnam and China Based on Fingerprint Combined with OPLS-DA[J].China Journal of Experimental Traditional Medical Formulae,2017,23(14):62-67. |
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| Authors: | GUO Wei SUN Rong WANG Liang ZHAN Zhi-lai JIN Yan YANG Bin and HUANG Lu-qi |
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| Institution: | School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Ji''nan 250355, China;Institute of Chinese Materia, China Academy of Chinese Medical Sciences, Beijing 100700, China;State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Shandong Academy of Chinese Medicine, Ji''nan 250014, China,Shandong Academy of Chinese Medicine, Ji''nan 250014, China,School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Ji''nan 250355, China;Shandong Academy of Chinese Medicine, Ji''nan 250014, China,State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China,Institute of Chinese Materia, China Academy of Chinese Medical Sciences, Beijing 100700, China and State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
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| Abstract: | Objective: To analyze the chemical differences of Smilacis Glabrae Rhizome from Vietnam and China, identify discrimination markers, and establish the method of identifying the origins of samples. Method: Samples were collected from Vietnam (7 batches) and China (28 batches). The chromatography fingerprints of the above samples were established, and the differences between the two kinds of samples were compared according to the similarity evaluation results. At the same time, the main chromatographic peaks were identified by HPLC-MS, and 21 peaks were identified. Cluster analysis was used to identify the origin of the samples. Orthogonal partial least squares discriminant analysis (OPLS-DA) was used to determine the discrimination markers between samples collected from Vietnam and China, and the points with VIP > 1 at the ends of S-plot were selected as potential markers. Result: Differences in main chemical compounds were found between the samples from Vietnam and China. Thirty-five samples were divided into Vietnam group and China group. Astilbin, isoastilbin, neoastilbin and engeletin were determined to be the discrimination markers, and their contents in samples of Vietnam were obviously higher than those in samples of China. Conclusion: Fingerprint coupled with OPLS-DA can be used to analyze the chemical similarity and differences between samples from Vietnam and China. The results provided data not only for the quality evaluation of samples from Vietnam, but also for the answer on whether samples from Vietnam can be used as medicine. |
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| Keywords: | Smilacis Glabrae Rhizome fingerprint OPLS-DA discrimination markers |
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