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DNA条形码技术在中药鉴定中的应用进展
引用本文:张彩云,黄珊珊,颜海飞.DNA条形码技术在中药鉴定中的应用进展[J].中草药,2017,48(11):2306-2312.
作者姓名:张彩云  黄珊珊  颜海飞
作者单位:广东食品药品职业学院 广东省中药研究所, 广东 广州 510520;广东食品药品职业学院 广东省中药研究所, 广东 广州 510520;中国科学院华南植物园 中国科学院植物资源保护与可持续利用重点实验室 广东省应用植物学重点实验室, 广东 广州 510650
基金项目:广东食品药品职业学院自然科学项目(2016YZ007)
摘    要:DNA条形码(DNA barcoding)技术利用标准的一个或多个DNA片段对物种进行鉴定,是近年来生物学研究的热点领域,也是生物学发展最迅速的方向之一。总结了植物DNA条形码研究的发展历程及最新进展,重点讨论了超级条形码在近缘物种鉴别中的应用前景,以及DNA条形码在中药材基原物种鉴定、道地药材鉴别以及中药材溯源系统研究中的应用,并强调了植物DNA条形码在中药资源评价、保护和可持续利用中的重要地位和作用,为药用植物DNA条形码研究提供新的思路。

关 键 词:DNA条形码  道地药材  分子鉴定  叶绿体基因组  超级条形码
收稿时间:2017/1/20 0:00:00

Applications of DNA barcoding in Chinese materia medica identification
ZHANG Cai-yun,HUANG Shan-shan and YAN Hai-fei.Applications of DNA barcoding in Chinese materia medica identification[J].Chinese Traditional and Herbal Drugs,2017,48(11):2306-2312.
Authors:ZHANG Cai-yun  HUANG Shan-shan and YAN Hai-fei
Institution:Guangdong Institute of Chinese Materia Medica, Guangdong Food and Drug Vocational College, Guangzhou 510520, China;Guangdong Institute of Chinese Materia Medica, Guangdong Food and Drug Vocational College, Guangzhou 510520, China;Key Laboratory of Plant Resources Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
Abstract:DNA barcoding involves the standardized use of one or a few DNA regions to tell species apart. It represents a hotspot of biological studies and one of the most rapid development directions of biology in recent years. Herein, we reviewed the development and current advances of plant DNA barcoding, focusing on the prospect of ultra-barcode in the identification of closely related species; The applications of DNA barcodes in the identification of traditional Chinese herbal medicine, the identification of genuine medicinal material and medicine traceability system. We also emphasized that the plant DNA barcoding will play an important role in the evaluation, protection and sustainable utilization of traditional Chinese medicine resources. This review may offer a new idea for the proceeding studies of medicinal plant DNA barcoding.
Keywords:DNA barcoding  genuine medicinal material  molecular identification  chloroplast genome  ultra-barcode
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