首页 | 本学科首页   官方微博 | 高级检索  
     


Noninsulin-like action of sodium orthovanadate in the isolated perfused liver of fed,non-diabetic rats
Authors:M. Roden  K. Liener  C. Fürnsinn  M. Prskavec  P. Nowotny  I. Steffan  H. Vierhapper  W. Waldhäusl
Affiliation:(1) Division of Endocrinology and Metabolism, Department of Internal Medicine III, University of Vienna, Austria;(2) Institute for Analytical Chemistry, University of Vienna, Austria
Abstract:Summary Vanadium compounds exert insulin-like effects on isolated rat adipocytes and skeletal muscle and improve glucose homeostasis in diabetic rats and mice. However, reports on metabolic actions of vanadium in the liver are still contradictory. Thus, the acute effect of sodium orthovanadate infusion on net glucose production was measured in isolated perfused livers of non-fasting, non-diabetic rats. Continuous infusion (0.2 ml/min; 90 min) of vanadate (10–500 mgrmol/l) rapidly increased hepatic glucose (p<0.001), but not cyclic AMP output, reaching peak values after 20 min. The cumulative glucose release displayed concentration dependence with a maximal net effect of 394.3 mgrmol/100 g body weight and an apparent half-maximal effective vanadate concentration of 19.6 mgrmol/l. The glycogenolytic response to vanadate was almost completely blocked by 100 mU/l insulin (p<0.005), by 0.1 mmol/l indomethacin (p<0.05) and in the absence of Ca2+ (p<0.001). These results indicate that sodium orthovanadate stimulates glycogenolysis in livers of fed, non-diabetic rats by a Ca2+-dependent mechanism, which may involve the release of prostaglandins.
Keywords:Vanadate  isolated liver perfusion  glycogenolysis  prostaglandins  insulin resistance
本文献已被 SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号