PTEN和p53在宫颈浸润癌和癌前病变组织中的表达及意义

目的探讨PTEN和p53在宫颈鳞癌组织及宫颈上皮内瘤变组织中蛋白表达及其意义。方法采用免疫组化SP法检测30例宫颈炎组织、50例宫颈上皮内瘤变(CIN)、30例浸润宫颈癌组织中PTEN基因和p53的表达。结果 PTEN在慢性宫颈炎、CIN Ⅰ、CIN Ⅱ、CIN Ⅲ、浸润性宫颈鳞癌组织中,阳性表达率分别为100%、92%、73.33%、40%、36.67%,表达率逐渐下降,差异有统计学意义(P<0.05)。PTEN表达与宫颈鳞癌病理分期有关(P<0.05),宫颈癌p53阳性的中PTEN缺失为70.83%,而p53阴性PTEN的缺失为61.54%,两者比较差异无统计学意义(P>0.05)。结论 PTEN和p53可能在宫颈癌的发生、发展中起重要作用,测定宫颈CIN中PTEN可作为宫颈癌的高危人群筛选指标,PTEN与p53检测还可作为宫颈癌的预后指标。

The expression and significance of PTEN and p53 in the tissues of invasive carcinoma of cervix uteri and cervical precancerous lesion

Objective To investigate the expression and significance of PTEN and 1）53 in the tissues of invasive carcinoma of cervix uteri and cervical precancerous lesion. Methods The expressions of P53 and PTEN protein were detected by immunohistochemistry method （SP） in 30 cases of chronic cervicitis,25 cases of cervical intraepithelial neoplasia （CIN I ）, 15 cases of CIN Ⅱ , 10 cases of CIN III,30 cases of invasive cervical squamous cell cancer （ 10 cases of stage I , 10 cases of stage Ⅱ, 10 cases of stage Ⅲ-Ⅳ ）. Results The positive expression rates of PTEN in chronic cervicitis, CIN Ⅰ , CIN Ⅱ , CIN Ⅲ and invasive cervical squamous cell cancer were 100%, 92%, 73. 33%, 40%, 36. 67%, respectively. The positive expression rates of PTEN in these specimens were in turn decreased,and there were significant differences among them （ P 〈0.05）. The expression of PTEN was correlated with the pathological staging of squamous carcinoma of the cervix （ P 〈 0.05 ）. The deletion rate of PTEN in p53 positive cervical carcinoma was 70.83% ,which was 61.54% in p53 negative cervical carcinoma, but there was no significant difference between them （ P 〉 0.05）. Conclusion PTEN and p53 may play an important role in the pathogenesis and development of cervical squamous cell cancer, and PTEN and p53 can be used as the indexes for screening high risk population of cervical cancer, which can also be used as the indexes for evaluating the prognosis of patients with cervical cancer.